Article Per Year (5 Year)
p-Index From 2020 - 2025
0.444
P-Index
This Author published in this journals
All Journal Research Journal of Life Science BUANA SAINS
Rosyida, Risya
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Mathematics Medicine & Pharmacology Physics

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Analysis of Chitinase Enzyme Trichoderma sp. in Degrading Fusarium oxysporum Rosyida, Risya; Martosudiro, Mintarto; Muhibuddin, Anton
Research Journal of Life Science Vol 9, No 3 (2022)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2022.009.03.5

Abstract

The chitinase enzyme in Trichoderma sp. play an important role in pathogen control. This is because chitin is the main component of the fungal cell wall which the chitinase enzyme can degrade. One Trichoderma sp. isolate was obtained (UBPK6) with the highest percentage of inhibition against Fusarium oxysporum, 76.71%. In addition, UBPK6 isolates also showed the highest bromocresol purple reagent test results (indicating the presence of chitinase enzymes). Chitinase enzyme activity was measured for Trichoderma asperellum isolates. The results showed that the optimum incubation period for enzyme production was the 4th day, with an enzyme activity value of 4.05 U/mL. It indicates that this time is the right time for harvesting enzymes. Furthermore, the effect of pH on the chitinase enzyme activity of Trichoderma asperellum fungus. The test results showed that the optimum value was produced at pH 5 with a value of 3.4 U/mL and decreased afterward. The pH five treatment was the best in inhibiting the growth of pathogens, with an inhibition value of 60.63%. The higher the content of the chitinase enzyme, the higher its ability to degrade damage to the germination of pathogenic spores, thus causing the growth of the pathogen to be inhibited.
MEKANISME ANTAGONISME BEBERAPA ISOLAT JAMUR ANTAGONIS TERHADAP PERTUMBUHAN JAMUR Colletotrichum gloeosporioides rosyida, risya
BUANA SAINS Vol 25, No 2 (2025)
Publisher : Universitas Tribhuwana Tunggadewi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33366/bs.v25i2.7328

Abstract

The pathogen Colletotrichum gloeosporioides causes anthracnose disease in cayenne pepper, which is difficult to control. It is expected to control the pathogen by using antagonistic fungi because it has the ability to stop its growth by means of antibiosis, parasitism, or competition. This study aims to test five isolates of antagonistic fungi for their ability to inhibit C. gloeosporioides, which causes anthracnose disease in cayenne pepper plants, and to identify the mechanism of inhibition in vitro. Inhibition and antagonistic mechanisms were used in the study. Each test used a full-randomized design. The antagonistic fungi used were Aspergillus sp., Penicillium sp., Gliocladium sp., Nigrospora sp., and Trichoderma sp., each repeated five times. The results showed that, compared to the other fungi, the antagonistic fungal isolate Trichoderma sp. had a high inhibitory ability (74%). The three isolates of antagonistic fungi have antagonistic mechanisms against C. gloeosporioides, namely Aspergillus sp., Gliocladium sp., and Trichoderma sp. In addition, isolates of Gliocladium sp., Nigrospora sp., and Trichoderma sp. also have parasitism ability, while Aspergillus sp., Penicillium sp., and Trichoderma sp. have antibiosis mechanisms against C. gloeosporioides. Antagonistic fungal isolates that have the most potential to be developed as biological agents for anthracnose control in cayenne pepper plants, namely Trichoderma sp.
Co-Authors Anton Muhibuddin Mintarto Martosudiro