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All Journal Tropical Animal Science Journal
Pazra, D. F.
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Agriculture, Biological Sciences & Forestry Energy

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Antidiabetic and Hypolipidemic Potential of Cow’s Milk Yogurt with the Addition of Herbal Extracts in Diabetic Rats Pazra, D. F.; Purwanti, M.; Handayani, K. S.; Wahyuningsih; Wahyuwardani, S.
Tropical Animal Science Journal Vol. 46 No. 4 (2023): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2023.46.4.494

Abstract

Yogurt, with the addition of herbal ingredients can add efficacy and value of its benefits. Several studies have proven that cinnamon, aloe vera, and fenugreek seeds have properties as antidiabetic and hypolipidemic. The purpose of this study was to evaluate the antidiabetic and hypolipidemic potential of yogurt with the addition of a combination of two or three types of herbal extracts, such as cinnamon, fenugreek seeds, and aloe vera, in diabetic rats. Diabetic rats were divided into six groups and given treatment for 21 days. Rats’ blood was taken to test blood glucose levels using glucometers, insulin with the ELISA method, blood lipid profiles, and kidney and liver functions using a chemistry analyzer. The data were statistically analyzed using one-way ANOVA followed by the Duncan test. The results showed that yogurt added with several combinations of herbal extracts such as cinnamon, aloe vera, and fenugreek seeds (III, IV, V, and VI) could significantly reduce blood glucose levels, increase insulin levels, improve lipid profiles, and improve kidney and liver functions in diabetic rats, as well as commercial administration of glibenclamide (II). Diabetic rats given yogurt with the addition of a combination of 3 herbal extracts (VI) and a combination of 2 herbal extracts (cinnamon +fenugreek seeds) (V) of 1% each showed better antidiabetic and hypolipidemic performances compared to the other groups (III, and IV). The combination of yogurt with some herbal extracts can add efficacy and value to the benefits of yogurt as a functional drink.
The Metagenomic Analysis of the Pig Digestive System Microbiome as a Basis for Disease Control on Farming in Tangerang District, Indonesia Endrakasih, E.; Pazra, D. F.; Wahyuningsih
Tropical Animal Science Journal Vol. 47 No. 3 (2024): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2024.47.3.280

Abstract

Characteristics of microbiome of the pig’s digestive tract play an important role in the animal’s physiology, including metabolism, nutrient processing, the body’s immune response, and disease resistance. This study aims to analyze the abundance and diversity of microorganisms (pathogenic and non-pathogenic) from the digestive system of pigs on farms in Tangerang District. The samples used in this research were pig feces from 43 pig farms in Tangerang District. Then DNA extraction was carried out using the Zymo Quick-DNA Fecal/Soil Microbe Kit according to manufacturing procedures. Sequencing was performed using an MGI DNBSEQ-G50 machine with a DNBSEQ-G50RS High-throughput sequencing set (FCL PE100) according to manufacturing procedures. The quality of fastq files was evaluated with FastQC. Taxonomic classification of the processed sequences was performed using Diamond, followed by MEGAN6. The microbiome of the pig’s digestive system was dominated by bacteria (85%) with the taxonomic profile at the phylum level of abundance dominated by Firmicutes (84.46%) and Bacteroidetes (9.11%). Abundance at the genus level was dominated by Enterococcus (20.44%) and Clostridium (10.31%), and at the species level it was dominated by Escherichia coli (4.92%) and Levilactobacillus brevis (4.84%). There were 10 species of pathogenic bacteria detected, with E. coli showing the highest relative abundance (4.92%). Changes in the gut microbiome play an important role in the physiology of animal health and disease. In addition, the pathogenic bacteria detected not only affect the health and productivity of pigs but also have the potential to threaten public health.
Co-Authors Endrakasih, E. Handayani, K. S. Purwanti, M. Wahyuningsih Wahyuwardani, S.