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All Journal Buletin Peternakan ANGON: Journal of Animal Science and Technology
Bata, Muhammad
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Immunology & microbiology Veterinary Other

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Kadar VFA Dan N-NH3 Domba Lokal Yang Diberi Pakan Mengandung Ekstrak Bunga Waru (Hibiscus tilisceus) Dengan Bahan Pembawa Yang Berbeda Secara In Vitro Purwanto, Tono; Bata, Muhammad; Rahayu, Sri
ANGON: Journal of Animal Science and Technology Vol 1 No 2 (2019): ANGON: Journal of Animal Science and Technology
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.angon.2019.1.2.p137-144

Abstract

Background. The purpose of the research was to study the effect of carrier and Hibiscus tiliaceus flower extract on VFA and N-NH3 rumen production of local sheep. Materials and Methods. The feed ingredients used were rice straw ammoniation and concentrate with dry matter ratio of 40:60. The method used experiment designed according to completely randomized design (CRD) of Factorial pattern of 2x3. There were two factors used in this research. The first factor was the carrier of rice bran (DP) and tofu byproduct (AT). The second factor were the three dosage of extract. Thereore, there were six combination used as treatments. Results. The results showed that there was an interaction tendency (P<0,057) between Hibiscus tiliaceus flower extract and carrier on VFA production. However it was not significant effect (P>0,05) on N-NH3 production. Both carrier and dousage had significant effect (P<0,05) on VFA levels, while, N-NH3 did not effect (P>0,05). Orthoghonal polynomial test showed that increasing of Hibiscus tiliaceus extract caused enhancing of VFA production quadratically and the highest VFA production achieved at 60 ppm with rice bran as carrier. Conclusion. The dosage of 60 ppm of Hibiscus tiliaceus extract with rice bran carrier produced highest of VFA production.
Fermentation Products, Microbial Protein Synthesis, and Hydrolytic Enzymes Activity of Rumen Fluids Local Beef Cattle Supplemented by Direct-Fed Microbials and Waru Leaf Flour Nursetiyadi, Dhany; Bata, Muhammad; Rahayu, Sri
Buletin Peternakan Vol 48, No 2 (2024): BULETIN PETERNAKAN VOL. 48 (2) MAY 2024
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v48i2.90556

Abstract

The aim of this study was to evaluate the effect of Waru Leaf Flour  (WLF) and Direct-Fed Microbials (DFM) supplementation on volatile fatty acid (VFA) and ammonia (N-NH3) concentrations, total protozoa, microbial protein synthesis (MPS), and the specific activity of cellulase, amylase, protease in rumen fluids of local beef cattle. The study was conducted as an experimental investigation using a factorial Completely Randomized Design (FCRD). The first factor were three levels of DFM (P) mixed with rice straw (0%, 0.5%, and 1% dry matter of rice straw). The second factor consisted of three levels of WLF supplementation (D) in concentrates (0%, 0.24%, and 0.48% of dry matter concentrate). This resulted nine treatment groups, each it replicated three times.The diet composition consisted of rice straw and concentrate at 40:60 ratio (% dry matter). The interaction between DFM and WLF had a statistically significant impact (p<0.01) on the rumen fluid's total protozoa, N-NH3, and protein content. However, this interaction was not statistically significant (P>0.05) in relation to VFA, MPS, cellulase, amylase, and protease activity. While no significant interaction were observed for total VFA, cellulase, amylase, and protease, DFM supplementation had a statistically significant effect (P<0.01) on reducing enzymes activity, and total VFA (P<0.05). SDS-PAGE and zymography analyzes showed two protease molecules at all treatments with molecular weight of 144 kDa and 133 kDa, cellulase molecules at 62-67 kDa and 19-21 kDa respectively. The addition of DFM showed a new protease band, they had a measurement of 14 and 25 kDa.This study provides valuable insights into enzyme activities in rumen, particularly protease, cellulase, and amylase. The results suggest that the combination of 0.24% WLF and 0.5% DFM supplementation holds promise for optimizing rumen conditions and improving the performance of local beef cattle.
Co-Authors Nursetiyadi, Dhany Purwanto, Tono SRI RAHAYU