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Antibacterial Retention of Star Anise (Illicium verum) Essential Oil and Oleoresin in the Cellulose-PEG Composite Miranda, Fitri Ariadna Sodi; Elvina Dhiaul Iftitah; Warsito
Indonesian Green Technology Journal Vol. 12 No. 1 (2023)
Publisher : Sekolah Pascasarjana, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.igtj.2023.012.01.01

Abstract

Star anise essential oil (EO) and oleoresin (OL) consists of various volatile compounds that can be easily damaged and affect their quality and function. The degradation is mainly caused by the deterioration and release of their active components. Encapsulating the extracts into a composite matrix can give slow release and minimize the contact between active compounds and surrounding. Cellulose-PEG composite has good compatibility and affinity, it can retain the extracts so does their biological activities. In this work, the essential oil and oleoresin were encapsulated into the cellulose-PEG composite with a ratio of 1:2 (µL EO and OL per mg of composite). The cellulose was obtained from extraction of star anise residue of EO and OL isolation. The extraction steps were pre-alkalization, alkalization, and three-stage bleaching. Antibacterial assay for the samples uses a combination of agar disc and well method, where the pure one uses the disc as absorbent and both the disc and composite are put in the well. The diameter of the inhibition zone against Staphylococcus aureus was observed once a week for eight week. The result shows that pure and composite-packed star anise essential oil and oleoresin samples have fluctuating antibacterial activity during the obeservation. Both packed EO and OL show a lower difference of the diameter of the inhibiton zone which indicated the composite can retain the antibacterial activity of the star anise essential oil and oleoresin. Keywords: Star anise, antibacterial, essential oil, oleoresin, composite.
Continuous Essential Oil and Oleoresin Extraction from Star Anise (Illicium verum) by Hydrodistillation and Solvent Extraction Miranda, Fitri Ariadna Sodi; Iftitah, Elvina Dhiaul; Warsito, Warsito; Berliana, Adilatunnisa Nur Indah; Seta, Fadhlih Arya
Indonesian Green Technology Journal Vol. 11 No. 2 (2022)
Publisher : Sekolah Pascasarjana, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.igtj.2022.011.02.03

Abstract

Star anise (Illicium verum) is widely used as a medicinal herb and spice. The extracts, essential oil and oleoresin, can be cici4d produced by hydro-distillation and steam distillation, while solvent and supercritical fluid extraction can be used to extract oleoresin. In this work, the star anise is distilled to obtain the oil, subsequently, its residue is extracted by solvent extraction to get the oleoresin. Whole and grounded star anise fruit is distilled by hydro-distillation for 8, 12, 16, 20, and 24 hours, thus the highest yield is obtained at 20 hours from grounded fruit, and the highest trans-anethole content of 68.50% is obtained at 8 hours from grounded fruit. Residue from the whole fruit-distillation process is extracted by Soxhlet extraction with three types of solvent (ethanol, diethyl ether, and n-hexane) for 6 hours. The highest yield was obtained from ethanol extraction with 1.16% yield and the highest content of trans-anethol was obtained from n-hexane fraction. One sample with the highest content of anethole from whole and grounded fruit-distilled oil and oleoresin are picked, and later they are tested to cici4d measure their antioxidant capacity by Ferric Reducing Antioxidant Power Assay (FRAP). Keywords: Essential oil, oleoresin, star anise, trans-anethole