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All Journal Jurnal Teknologi Pangan dan Hasil Pertanian AgriHealth: Journal of Agri-food, Nutrition and Public Health
Ismaya, Pandu Legawa
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Agriculture, Biological Sciences & Forestry Computer Science & IT Education Environmental Science Health Professions Public Health

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Perubahan Mutu Buah Pepaya Varietas IPB 9 (Calina) Selama Penyimpanan Pasca Simulasi Transportasi Ismaya, Pandu Legawa; Faturochman, Hadi Yusuf; Darmawati, Emmy; Setyadjit, Setyadjit
Jurnal Teknologi Pangan dan Hasil Pertanian Vol. 18 No. 2 (2023): September
Publisher : Faculty of Agricultural Technology, Universitas Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26623/jtphp.v18i2.7746

Abstract

Papaya (Carica Papaya L.) IPB 9 (calina) variety is a papaya fruit that has thick, sweet flesh and very high fruit productivity. During the transportation process of papaya, mechanical damage (bruises, abrasions, weight loss) and physiological damage often occur, which can cause papaya decrease in quality during storage before consumption. The purpose of this study was to determine changes in the quality of papaya after 2-hour transportation simulation. The research design used a randomized block design (RAK) with the treatment of the position of the fruit in the packaging, which was stored horizontally (KS1) and vertically (KS2) during the transportation simulation process. Papaya hat has been simulated for transportation is stored at 18–20oC for 12 days. Quality of papaya weight loss stored for 12 days for KS1 reached 7.34% and KS2 reached 7.43%. Hardness quality of papaya  stored for 12 days for KS1 is 0.84 kgf, and for KS2, it is 0.76 kgf. Quality of papaya skin color after 12 days of storage decreased the freshness of the papaya fruit, but it was still fit for consumption. Total dissolved solids content after being stored for 12 days for KS1 reached 11.45% and KS2 reached 11.32%.
Prevalence and Molecular Detection of Toxigenic Genes of Bacillus cereus in Rice-Based Street Foods in Tasikmalaya, Indonesia Maerani, Maerani; Ghaffar, Mufti; Munggarani, Fadhiya; Nuraeni, Dina Aulia; Ismaya, Pandu Legawa; Faturochman, Hadi Yusuf
AgriHealth: Journal of Agri-food, Nutrition and Public Health Vol 7, No 1 (2026): April
Publisher : Research and Development Center for Food, Nutrition and Public Health (P4GKM) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agrihealth.v7i1.106053

Abstract

Bacillus cereus is a spore-forming foodborne pathogen frequently associated with rice-based foods and capable of causing emetic and diarrheal illnesses through the production of heat-stable and heat-labile toxins. This study investigated the prevalence, contamination levels, and toxigenic gene profiles of B. cereus in rice-based street foods. A total of 12 samples, including fried rice, chicken porridge, and nasi kuning, were collected from street vendors in Kahuripan Sub-district, Tasikmalaya, Indonesia. Presumptive B. cereus was isolated using mannitol yolk polymyxin agar (MYPA) as a selective medium and confirmed biochemically and molecularly by PCR targeting the gyrB gene. Toxigenic potential isolate was evaluated by detecting the nheA, hblD, cytK, entFM, and ces genes. Presumptive B. cereus was detected in 91.67% (11/12) of samples, while molecular confirmation identified gyrB-positive isolates in 42% (5/12) of samples, originating from fried rice and chicken porridge. No significant association was found between food type and gyrB detection (p > 0.05). Contamination levels ranged from < 1.5 × 10¹ to 2.95 × 10⁶ CFU g-1. All confirmed isolates harbored at least one toxigenic gene, with nheA being the most prevalent (80%), followed by cytK (60%), entFM (40%), ces (40%), and hblD (20%). Notably, one of the fried rice isolates carried all 5 toxin-related genes, indicating a high virulence potential. These findings demonstrate that rice-based street foods in Tasikmalaya may pose a significant public health risk due to contamination with toxigenic B. cereus. Enhanced hygiene practices, strict temperature control, and molecular surveillance are essential to reduce the risk of foodborne illness associated with street-vended rice products.
Co-Authors Emmy Darmawati Faturochman, Hadi Yusuf Ghaffar, Mufti Hadi Yusuf Faturochman Maerani, Maerani Munggarani, Fadhiya Nuraeni, Dina Aulia Setyadjit Setyadjit, Setyadjit