Article Per Year (5 Year)
p-Index From 2021 - 2026
0.817
P-Index
This Author published in this journals
All Journal Jurnal Pengabdian Masyarakat Asia Jurnal Ilmiah Wahana Pendidikan Halal Research Journal Jurnal Serambi Biologi
safitri, fira
Unknown Affiliation
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Engineering Immunology & microbiology Social Sciences Other

Published : 4 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search
Journal : Halal Research Journal

 1 
Optimization of Authentication Methods for Processed Chicken Meat Products Based on ND5 Gene qPCR Achyar, Afifatul; Safitri, Fira; Atifah, Yusni; Farma, Siska Alicia; Putri, Dwi Hilda; Ahda, Yuni
Halal Research Vol 5 No 2 (2025): July
Publisher : Halal Center ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j22759970.v5i2.1823

Abstract

Consumers have the right to correct information about the products they consume. One example of a food product that is vulnerable to counterfeiting is chicken nuggets. To overcome this problem, qPCR can be an effective solution. Previous research has designed primers targeting the chicken ND5 gene. However, the PCR conditions for this primer pair have never been optimized. This research aims to optimize qPCR conditions and carry out chicken nugget authentication trials. Genomic DNA was isolated from five weight variations of chicken meat samples as a qPCR standard curve and three chicken nugget samples. Optimizing the annealing temperature with gradient PCR gave the best results at a temperature of 57.6OC. The results of the authentication test showed that the chicken meat content in TD nuggets was 54.7%, C nuggets 43.8%, and D nuggets 37.3%. This value is still in accordance with the Indonesian National Standard (SNI).
Co-Authors Achyar, Afifatul Atifah, Yusni Br Panjaitan, Andra Wina Dwi Hilda Putri Jaizna, Muhammad Nabiel Mardalena Hanifah Maryati Bachtiar Novriano, Rama Permatasari, Mutiara Rahman, Azra Aulia Samariadi, Samariadi Yulisa, Farah Amalia Yuni Ahda