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All Journal Jurnal Biologi Tropis Jurnal Serambi Biologi
Chahyunisa, Alifia
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Growth of Trichoderma asperellum with the Addition of Glycerol on Rice Bran Based Medium Chahyunisa, Alifia; Anhar, Azwir
Jurnal Serambi Biologi Vol. 9 No. 1 (2024): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/srmb.v9i1.309

Abstract

Biofertilizer is a material that contains live microorganisms that are able to increase plant nutrition, one of which is Trichoderma. Trichoderma is able to have a positive influence on plants, plant growth and plant production results. Bran is a material that can be used as a growth medium for Trichoderma sp because it is able to increase nutrition in plants and is able to meet the needs needed for the growth and development of Trichoderma. The shelf life of bran is limited, therefore glycerol is added to the bran medium. This research is an experimental study using a Completely Randomized Design (CRD) with 6 treatments and 5 replications. The research was carried out by making a Trichoderma asperellum formulation using bran as the basic ingredient and adding glycerol at concentrations namely 0%, 20%, 40%, 60%, 80% and 100%. Data in the form of the number of spores at each concentration were analyzed using analysis of variance. The results of the research showed that for Trichoderma asperellum, giving different concentrations of glycerol in bran-based media did not affect the number of Trichoderma asperellum spores and shelf life because there was no real difference between the different concentrations given.
Addition of Glycerol as A Growth Stabilizer for Trichoderma asperellum in Bran-Based Growth Media Chahyunisa, Alifia; Anhar, Azwir
Jurnal Biologi Tropis Vol. 25 No. 2 (2025): April-Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i2.8724

Abstract

Biofertilizer is a fertilizer that contains microorganisms that increase plant fertility and reduce environmental pollution. One of the microorganisms that can be used as a biofertilizer is Trichoderma asperellum. Trichoderma can be cultured in several growth media, one of which is bran. In an effort to extend the shelf life of Trichoderma, glycerol is added as a stabilizer.This research aims to see how the addition of glycerol affects the shelf life of Trichoderma asperellum. This reserach is experimental research. Data were analyzed  using a completely randomized design (RAL). The treatment given in this research was by adding glycerol to the T. asperellum growth medium. T. asperellum cultures which were cultured in liquid bran-based media which were given glycerol and which not given glycerol were observed for spore growth, then the spore density was counted using a haemocytometer every 15 days. The research results obtained were that T. asperellum was able to survive in the growth medium for 45 days and had no real effect because it was unable to maintain the number of T. asperellum spores.
Co-Authors Azwir Anhar