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All Journal HAYATI Journal of Biosciences Indonesian Journal of Dental Medicine Academia Open The Indonesian Journal of Community and Occupational Medicine
Alwan, Maryam Hameed
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Agriculture, Biological Sciences & Forestry Arts Humanities Chemistry Computer Science & IT Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Health Professions Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Medicine & Pharmacology Physics Social Sciences Other

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Histological and Molecular Evaluation of the Antiproliferative Activity of Allium ampeloprasum Water Extract Against Oral Mucosa Cell Line (Gingival Cancer) Alwan, Maryam Hameed; Hameed, Zainab; Mandala, Satria
HAYATI Journal of Biosciences Vol. 31 No. 5 (2024): September 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.5.829-835

Abstract

Gingival carcinoma is a malignant neoplasm affecting the oral mucosa and is associated with significant morbidity and mortality. Allium ampeloprasum var. porrum water extracts have gotten a lot of attention because of their bioactive components, such as polyphenols, flavonoids, and alkaloids, which have a variety of pharmacological activities, including antiproliferative actions. This study aimed to evaluate the histological and molecular effects of Allium ampeloprasum (leek) water extract on the proliferation of the murine gingival cancer cell line. Histological evaluation was conducted to examine morphological changes induced by extract treatment. Molecular mechanisms underlying the observed histological changes were investigated using real-time polymerase chain reaction (PCR). Expression levels of key genes associated with cell proliferation and apoptosis were assessed. Histological findings revealed a dose-dependent decrease (100, 50, 25, 12.5, and 6.25 µg/ml) in cell density and altered cell shape in the treated cell line. Also, the percentage of inhibition for the oral mucosa cell line was high, with a significant P of 0.006, in the treated group compared to the control group. Additionally, water extract has an IC50 value of 61 g/ml. The P53 fold increment of gene expression is 0.6, which means the expression level in the experimental condition is 60% higher than the control. This study provides evidence for the potential antiproliferative activity of Allium ampeloprasum water extract on the oral mucosa cell line. The observed histological changes, coupled with the modulation of key genes involved in proliferation and apoptosis, suggest that leek water extract may have therapeutic implications in managing oral cancer.
Impact of COVID-19 on Dental Students' Psychological Health Alwan, Maryam Hameed
Academia Open Vol 8 No 2 (2023): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/acopen.8.2023.8087

Abstract

This study investigates the psychological impact of the COVID-19 pandemic on dental students at Baghdad University College of Dentistry. Conducted between December 2021 and January 2022, this cross-sectional survey aligns with ethical guidelines and the Helsinki Declaration. The study utilized Cochran's equation to determine a sample size of at least 400, ensuring a 95% confidence level with a 5% margin of error. The Perceived Stress Scale (PSS) and the Covid Student Stress Questionnaire (CSSQ) were employed as primary tools, assessing general and COVID-19-related stress, respectively. A total of 411 students participated, with 67.50% experiencing moderate to severe stress (PSS <14) and 58.8% reporting average levels of COVID-related stress (CSSQ <7). Notably, there was a significant positive correlation between the PSS and CSSQ scores (P = 0.008). The analysis, conducted using IBM SPSS Statistics software V26, included descriptive statistics, Cronbach's alpha for reliability, and Pearson Correlation for assessing correlations. The findings indicate that a substantial proportion of dental students experienced heightened stress levels, potentially leading to mental health disorders like cognitive impairment. These results emphasize the need for universities to enhance psychological support and for government bodies to implement comprehensive health education and awareness programs. This study not only sheds light on the immediate psychological effects of the pandemic on dental students but also serves as a foundational reference for future interventions aimed at reducing stress levels in this demographic. Highlights: Dental students have experienced significant psychological impacts from the COVID-19 pandemic. The pandemic has caused increased stress, anxiety, and depression among dental students. Remote learning and decreased clinical experience have contributed to the negative psychological effects. Providing social and emotional support is important in mitigating the psychological impact of the pandemic on dental students. Keywords: COVID-19, SARS-CoV-2, Student, Dental, Stress
Observance of the Adherence to Antibiotic Prescriptions by Iraqi Dental Patients: A Different Perspective on Antimicrobial Resistance Hatem, Mohammed Khamas; Alwan, Maryam Hameed
The Indonesian Journal of Community and Occupational Medicine Vol. 4 No. 3 (2025): ijcom
Publisher : ILUNI MKK FKUI and PRODI MKK FKUI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53773/ijcom.v4i3.150.94-8

Abstract

Background: The excessive use and abuse of antibiotics contribute to bacterial resistance, raising the risk of complications and treatment failures. This study investigates adherence to antibiotic prescriptions among Iraqi dental patients, highlighting implications for antimicrobial resistance.Objective: To assess adherence levels and identify factors influencing antibiotic therapy compliance among dental patients.Methods: A cross-sectional survey was conducted in which adult dental patients aged 18 and older, who had been prescribed antibiotics within the past year, participated. The modified Morisky Medication Adherence Scale-8 items was used to evaluate adherence, and data were analyzed with IBM SPSS Statistics software V26.Results: Among 100 participants, 56% reported forgetting to take their antibiotics, 45% intentionally skipped doses, and 53% reduced or halted their doses. Adherence levels were categorized as medium in 45%, low in 28%, and complete in 27%. There were no significant differences by gender; however, adherence varied significantly across age groups, being higher in those aged 39-59.Conclusion: The study underscores the need for targeted interventions to improve adherence to antibiotic therapy among dental patients, which is essential for mitigating antimicrobial resistance and enhancing treatment outcomes.
Impact of VAPE use on dental and oral health among male dental students Khalaf, Mohammed Hassan; Alwan, Maryam Hameed
Indonesian Journal of Dental Medicine Vol. 8 No. 1 (2025): Indonesian Journal of Dental Medicine
Publisher : Faculty of Dental Medicine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijdm.v8i1.2025.1-5

Abstract

Background: The emergence of vaping as a popular alternative to traditional smoking has raised concerns regarding its potential impact on oral health. specifically affects the oral health of dental students, who are not only consumers of these products but also future practitioners tasked with educating patients on oral hygiene and health risks. purpose: This study investigated the dental and oral health impact of VAPE among male dental students. Methods: A self-administered questionnaire comprising 20 questions was meticulously designed to gather relevant data about their VAPE use and its implications on dental and oral health. Results: 43% of participants reported using VAPE, predominantly employing salt nicotine (48.8%) over free base nicotine (27.9%). The dental health statuses, assessed using the Decayed, Missing, and Filled Teeth (DMFT) index, showed that 35.6% had a DMFT score of 3 or higher, while 37.6% had scores below 3, and 26.7% reported a DMFT of 0. Most participants (57.4%) visited the dentist rarely, with no distinction between VAPE users and non-users. However, significant differences in aspects such as breathing, sleep quality, and fitness were noted between VAPE users and non-users (p:0.002, p:0.05, and p:0.00; p<0.05), respectively. Conclusion: As E-cigarettes smoking is becoming more widely growing habit within the young age group, more oral and physiological consequences begin to rise, such as a significantly higher prevalence of mouth dryness, cough and ultimately, worsened bodily functions including a lower breathing quality and reduced level of fitness.
Co-Authors Hameed, Zainab Hatem, Mohammed Khamas Khalaf, Mohammed Hassan Satria Mandala