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Anatomical Responses of Bambusa blumeana Schult.f. Treated with Alpha-Naphthalene Acetic Acid Saddoy, John Karlo Cruz; Tinio, Crusty Estoque; Aboc, Vinjoe Magalang; Luna, Amelita Carpio; Combalicer, Marilyn Sabalvaro; Maldia, Lerma San Jose
Jurnal Sylva Lestari Vol. 12 No. 3 (2024): September
Publisher : Department of Forestry, Faculty of Agriculture, University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jsl.v12i3.973

Abstract

The underlying mechanism of bamboo anatomical responses remains poorly understood when subjected to different levels of Alpha-Naphthalene Acetic Acid (Alpha-NAA) under nursery conditions. In this paper, we aim to examine the leaf and root anatomy of the Bambusa blumeana Schult.f., a potential bioremediation alternative species, treated with 0 ppm, 100 ppm, and 200 ppm of Alpha-NAA. The experimentation was executed in a completely randomized design within a 3-month experimentation period. Freehand sectioning was performed to collect the leaf and root transverse sections, and the photomicrographs were taken and analyzed. The results revealed that 200 ppm Alpha-NAA significantly increased the diameters of leaf fusoid cells and xylem. In the roots, the thickness of the rhizodermis, sclerenchymatous layer, cortex, and pericycle, as well as the diameter of the phloem, metaxylem, and pith, were significantly affected by the treatments. Generally, there is a weak to moderate correlation between the leaf and root anatomical structures based on Pearson’s correlation and a weak to strong correlation within the organ-specific anatomical structure. Thus, our findings elucidate the potential role of exogenous auxin in inducing anatomical changes that assist the growth and propagation of B. blumeana. Keywords: alpha-naphthalene acetic acid, bamboo, freehand sectioning, leaf anatomy, root anatomy
Native Metallophytes on Ultramafic Wooded Grassland in Sta Cruz, Mindoro Occidental, Philippines: Insights Into Phytostabilization and Forest Restoration Quimado, Marilyn Ong; Hernandez, Jonathan Ogayon; Tinio, Crusty Estoque; Cambel, Maria Patrice Salazar; Luna, Amelita Carpio; Fernando, Edwino Sanson
SAINS TANAH - Journal of Soil Science and Agroclimatology Vol 20, No 2 (2023): December
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/stjssa.v20i2.65592

Abstract

The native metallophytes species are the optimum choice to restore degraded areas on ultramafic soil. However, a limited restorative floristic survey on the wooded grassland of Mindoro Occidental had been reported. Four 20 m x 20 m plots were established to rapidly assess the plant diversity of a wooded grassland on ultramafic soil in Sta. Cruz, Mindoro Occidental, Philippines. Diversity index (H`), relative density, relative dominance, and importance value (IV) were computed. Physicochemical characteristics and heavy metal contents of the soil in the site were analytically determined. We identified 43 morpho-species of plants belonging to 25 families. Thirty-six of the morpho-species identified are Philippine natives and typically grow on ultramafic forests. Nine species out of the top 10 trees with the highest IV are native ones, with Buchanania arborescens Blume as the most dominant. The estimate of Relative Cover (%) also showed native species. Poles and sapling dominated the area, suggesting that active regeneration is taking place. Further, 72.94% of the ground cover were represented by tree flora recruits (e.g., B. arborescens, Alstonia macrophylla). The sites have overall moderate diversity (H` index of 2.7). The soil contains a high amount of Nickel, Chromium, Iron, and Manganese. There were no Nickel hyperaccumulators but 22 native species showed Aluminum and Silicon hyperaccumulation. Therefore, the study revealed that the surveyed area is home to important metallophytes that have the potential for phytostabilization and reforestation.