<![CDATA[growth and development of anthers of two soybean cultivars, Merubetiri and Wilis cultured in vitro. The trial was conductedat the Plant Biotechnology Laboratory, Agricultural Faculty, University of Jambi. Anthers were cultured on MS solidmedium provided with IAA, 2,4-D or NAA as auxin source in combination with BAP or kinetin as cytokinin source. Eachgrowth regulators was tested at 0, 5, 10, 15 and 20 μM. The experiment was placed in a completely randomized design withfive replicates. Each replicates consisted of 8 to 10 anthers obtained from the same floral bud. Cultures were placed in a lightintensity of 50 μmol m-2.s-1 and 16-hour photoperiod at 25±1 oC. Observation was done weekly for 8 weeks of culture.Results indicated that response showed by anthers cultured on medium supplemented with 2,4-D+BAP, IAA+BAP andNAA+BAP, in the form of callus proliferation, occurred within 5-18 days of culture initiation. Meanwhile, with 2,4-D+kinetin,IAA+kinetin and NAA+kinetin, callus proliferation took place within 4-16 days of culture initiation. Callus formation waspreceded by a swollen on the surface of anthers, followed by changing in color from light green to brownish. Following this,anther wall turned into amorphous shape, before it was finally covered by a white, cream or light green callus mass. Initially,the callus showed friable or compact structure, but following two weeks of proliferation all callus showed compact structure.Among growth regulators tested, combination involving 2,4-D produced more callus than other combinations. In addition,of the two cultivars tested, Merubetiri showed better response compared to Wilis.]]>
