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All Journal Journal La Lifesci Nabatia
Ghanim, Nadhim Salim
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Agriculture, Biological Sciences & Forestry Chemistry Earth & Planetary Sciences Environmental Science Immunology & microbiology

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Using RAPD-PCR to Determine the Genetic Stability of Dianthus Chinesis in Vitro Treated with the Chemical Mutagenic Ethyl Methyl Sulfonate EMS Jayed, Ghufran Adnan; Alsugmiany, Rafea Zaidan Mukhlif; Ghanim, Nadhim Salim
Journal La Lifesci Vol. 3 No. 5 (2022): Journal La Lifesci
Publisher : Newinera Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37899/journallalifesci.v3i4.815

Abstract

This study was conducted in the Molecular Biology Lab. and the Central Lab. at the University of Tikrit on 1/29/2023 for the purpose of determining the genetic stability of the Dianthus chinesis after cultivating it in vitro using 3 RAPD primers, which are OPA-02, OPD-10, OPD- 18, As 9 samples were used in the study, the first is a field sample of this plant that was not treated with EMS (was considered as a control sample) and the remaining samples (4 callus samples and 4 tissue branches samples) were treated with concentrations of (0.0, 0.2, 0.4, 0.6)% of EMS, after a month, DNA was extracted, RAPD reactions were performed for the primers used ,as it was shown through the results that there was a genetic variation in the genetic material of the Dianthus chinesis in the recognition sites of the used primers, as it was not only affected by EMS, but by its cultivation in vitro.
Induction Callus From Cotyledons of Atropa belladonna L. and Evolution Ionic Content Using Plant Tissue Culture Ahmed, Ekhlas Meteab; Ghanim, Nadhim Salim; Aziz, Wijdan Saadi
Nabatia Vol 13 No 1 (2025): June
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/nabatia.v13i1.1655

Abstract

This study dealt with the induction of callus tissue of the belladonna plant Atropa belladonna L. through the establishment of tissue cultures by growing cotyledons on Whites-(1934) medium (WM) supplied with different concentrations of BAP (0.0, 5.0, and 1.5) mg.L-1 and 2 4-D at level (0.0, 1.5, and 2.0) mg.L-1, Callus tissue implantation at different levels of Salicylic acid, tyrosine, Jasmonic acid, PEG, and NaCl separately. The results showed that the best fresh and dry weight was obtained from the induction of callus when adding BAP At level 1.5 mg.L-1 mixed with 2,4- D At level 2.0 mg.L-1 reached 3.97 g and 0.649 mg on straight. The results showed the highest percentage of nitrogen reached 0.98 µg.gm dry weight when adding Jasmonic acid at level 75 mg.L-1, and the highest percentage of phosphorus reached 0.67 µg.gm dry weight when adding PEG at level 0.5 gm, and the highest percentage of potassium reached 0.98 µg.gm dry weight when adding Salicylic acid at a concentration of 75 mg.L-1 , and the highest increase in calcium reached 637.0 µg.gm dry weight when adding Jasmonic acid at level 75 mg.L-1 .and the highest percentage of sodium reached 185.3 µg.gm dry weight when adding tyrosine at level 40 mg.L-1, and the highest increase in chlorine reached 178.2 µg.gm dry weight when adding NaCl at level 75 mg.L-1.
Co-Authors Ahmed, Ekhlas Meteab Alsugmiany, Rafea Zaidan Mukhlif Aziz, Wijdan Saadi Jayed, Ghufran Adnan