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All Journal Padjadjaran Journal of Dentistry JITEKGI Jurnal Ilmiah dan Teknologi Kedokteran Gigi Jurnal Kedokteran Gigi Terpadu
Berliana, Stefhanie
Unknown Affiliation
Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Aggregatibacter actinomycetemcomitans dan Porphyromonas gingivalis: Bakteri Destruksi Tulang Alveolar Berliana, Stefhanie; Octarina
Jurnal Kedokteran Gigi Terpadu Vol. 6 No. 1 (2024): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jkgt.v6i1.20817

Abstract

The oral cavity is a suitable habitat for various types of bacteria, including Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis. Both of these bacteria can damage periodontal tissues, including alveolar bone, through various mechanisms. Prevention against these bacteria can be accomplished by maintaining oral hygiene. The purpose of this literature review was to provide information about bacteria that can cause damage to alveolar bone and ways to prevent it. The results of this literature review would provide information about the mechanisms of Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis bacteria in causing alveolar bone damage, as well as ways to prevent these bacteria.
Antibacterial and cytotoxic effects of fresh bovine amniotic membrane with hydroxyapatite (BAM-HA): a laboratory experiment Octarina, Octarina; Berliana, Stefhanie; Kalangit, Ruth Belatriks
Padjadjaran Journal of Dentistry Vol 36, No 1 (2024): March 2024
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol36no1.53128

Abstract

Introduction: Bacterial infections, particularly by Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) and Porphyromonas gingivalis (P. gingivalis), can worsen alveolar bone resorption after tooth extraction. The capability of Bovine Amniotic Membrane-Hydroxyapatite (BAM-HA) biocomposite to reduce this resorption has been explored. However, before clinical use, cytotoxicity testing is imperative to ensure its biocompatibility. The aim of the study was to assess both the antibacterial effects and cytotoxicity of the BAM-HA biocomposite to ensure its suitability for clinical use biocompatibility of the BAM-HA biocomposite before its clinical application. Methods: The laboratory-based research involved testing BAM combined with HA powder in 4:1 and 4:2 ratios via freeze-drying and underwent antibacterial tests against A. actinomycetemcomitans and P. gingivalis, using the plate count method. Cytotoxicity tests were performed on HGF cells, including negative control, positive control, BAM-HA (4:1), and BAM-HA (4:2) groups, with statistical analysis conducted using One-Way ANOVA and Post Hoc Bonferroni and Tukey tests. Results: Antibacterial tests against A. actinomycetemcomitans revealed significant reduction in colony count with BAM-HA ratios 4:1 (129.0 ± 12.7 CFU/mL) and 4:2 (77.3 ± 15.5 CFU/mL) compared to the negative control (186.6 ± 27.5 CFU/mL). Similar reductions were observed for P. gingivalis, with BAM-HA ratios 4:1 (51.3 ± 6.6 CFU/mL) and 4:2 (3.1 ± 1.5 CFU/mL) compared to the negative control (117.3 ± 22.0 CFU/mL). Cytotoxicity tests showed no significant differences in HGF cell viability and IC50 values between the negative control and BAM-HA (4:1) or BAM-HA (4:2) groups. Conclusion: The BAM - HA biocomposite shows antibacterial effects against A. actinomycetemcomitans and P. gingivalis. Moreover, BAM - HA ratios of 4:1 and 4:2 do not induce cytotoxic effects on human gingival fibroblasts, suggesting potential biocompatibility for clinical applications.KeywordA. actinomycetemcomitans, antibacterial effects, BAM-HA biocomposite, cytotoxicity, P. gingivalis
GAMBARAN PERUBAHAN LAJU DEGRADASI BIO-OSS COLLAGEN SEBAGAI MATERIAL PENGGANTI TULANG ALVEOLAR Octarina, Octarina; Berliana, Stefhanie; Falatehan, Niko
Jurnal Ilmiah dan Teknologi Kedokteran Gigi (JITEKGI) Vol 20, No 2 (2024)
Publisher : Fakultas Kedokteran Gigi Universitas Prof. Dr. Moestopo (Beragama)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32509/jitekgi.v20i2.4055

Abstract

Latar belakang: Perubahan dimensi tulang alveolar setelah pencabutan gigi, baik lebar maupun tinggi dapat mempengaruhi fungsi prostetik dan keberhasilan perawatan implan. Salah satu biomaterial yang telah banyak digunakan secara komersil untuk mengatasi masalah ini adalah Bio-Oss Collagen. Material ini diaplikasikan setelah pencabutan gigi untuk mencegah terjadinya resorpsi tulang alveolar serta merangsang regenerasi pembentukan tulang alveolar yang baru. Bio-Oss Collagen sering dipilih sebagai pengganti tulang karena memiliki biokompatibilitas yang sangat baik. Tujuan penelitian ini adalah menganalisis sifat biodegradasi dari material Bio-Oss Collagen untuk menilai efektivitasnya dalam mendukung regenerasi tulang alveolar dan mempertahankan struktur tulang yang optimal. Metode Penelitian: Bio-Oss Collagen ditimbang untuk mendapatkan berat awal. Material kemudian direndam dalam Larutan SBF (Simulated Body Fluid) selama 7 hari. Setelah itu, material tersebut diproses dengan  freeze drying pada suhu -100°C selama 24 jam. Proses penimbangan kembali dilakukan untuk mendapatkan berat akhir material setelah perendaman dan freeze drying. Hasil: Penelitian ini menunjukkan bahwa dalam waktu 7 hari, Bio-Oss Collagen dapat terdegradasi dengan mudah dan meninggalkan granul demineralized bone freeze-drying. Laju rata-rata biodegrasi dari material Bio-Oss Collagen adalah sebesar 3,66%. Kesimpulan: Laju biodegradasi dari Bio-oss Collagen dengan persentase 3,66% menunjukkan bahwa Bio-Oss Collagen memiliki sifat biokompatibel dan dapat digunakkan sebagai material pengganti tulang alveolar. Kemampuan biodegradasi Bio-Oss Collagen yang efisien dapat memfasilitasi proses regenerasi jaringan tulang dan memperbaiki serta mempertahankan struktur yang optimal.
Co-Authors Kalangit, Ruth Belatriks Niko Falatehan Octarina