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Identifikasi Endoparasit pada Feses Lembu (Bos taurus) di Desa Tuntungan 2 Kecamatan Pancur Batu Deli Serdang Provinsi Sumatera Utara Augustin Ningrum, Nurlian; Wahyuni, Rana; Aulia Puspita, Rizka; Aprilia, Lola; Aulia Pohan, Dinda
Journal of Multidisciplinary Inquiry in Science, Technology and Educational Research Vol. 1 No. 4 (2024): AGUSTUS-OKTOBER 2024
Publisher : UNIVERSITAS SERAMBI MEKKAH

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32672/mister.v1i4.2224

Abstract

Various types of diseases can occur on farms. One of the diseases in livestock is caused by infection with parasites and parasitic worm eggs. This parasite can attack the stomach organ tissue in cattle and can even spread to other organ tissue such as the liver, lungs, and even the brain. The aim of this research was to identify various types of parasites and estimate their occurrence in cow dung in Tuntungan 2 Village, Pancur Batu District, Deli Serdang Regency, North Sumatra Province. Twenty samples of cow dung were used. Sampling was carried out randomly, by collecting 10 grams of feces at each location. Floating, Sedimentation, and Mc Master methods are used in the identification process. apply parasite prevalence models to data analysis calculations. Research findings show that parasitic worms are found in animal feces in Tuntungan 2 Village. There are 3 types of worms found, namely: Trichostrongylus sp. Bunostomum sp and Oesophagostum sp. Meanwhile, 4 types of worm eggs were found, namely Eimeria sp, Oesophagostum sp, Bunostomum sp, and Trichostrongylus sp. T1 sample location is 0.45% which is more dominant. The prevalence of infection is quite high. With the degree of infection of all species in the heavy category, the degree of infection was most often found at the T1 sample location which had a very high number of parasitic worm eggs.
Pengaruh Pemberian Ekstrak Buah Gandaria (Bouea macrophylla Griff) Terhadap Perubahan Ekspresi Gen CD36 Pada Otak Tikus Yang Diinduksi CCL4 (Karbon Tetraklorida) Wahyuni, Rana; Idami, Zahratul
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.19356

Abstract

This study aimed to analyze the effect of gandaria fruit extract (Bouea macrophylla Griff.) on CD36 gene expression in the brains of CCl₄-induced rats (Rattus norvegicus). The experiment employed a completely randomized design consisting of five groups: negative control, positive control (CCl₄), and three treatment groups administered gandaria fruit extract at doses of 100, 150, and 200 mg/kgBW. CD36 gene expression was assessed using semi-quantitative PCR, normalized to the GAPDH reference gene, and analyzed based on band density using ImageJ software. Statistical analysis was performed using one-way ANOVA followed by post hoc testing at a significance level of p < 0.05. The results demonstrated that CCl₄ induction increased CD36 expression by 1.9-fold compared to the control group. Administration of gandaria fruit extract reduced CD36 expression relative to the CCl₄ group, with the lowest fold change observed at 100 mg/kgBW (1.08), while higher doses showed relatively increased expression but remained lower than the positive control. These findings indicate that gandaria fruit extract has the potential to modulate CD36 gene expression under CCl₄-induced oxidative stress conditions, with the most effective response observed at the lowest dose.