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All Journal JMGCB Journal of Medical Genetics and Clinical Biology (JMGCB)
Halboti, Alaa Abdalhadi
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Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing

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UNRAVELING THE ROLE OF EPIGENETIC MODIFICATIONS IN GENE REGULATION A MOLECULAR GENETICS PERSPECTIVE AL-Abedi, Yasameen Waleed; Halboti, Alaa Abdalhadi; Saleh, Ali Abdulhussein
Journal of Medical Genetics and Clinical Biology Vol. 1 No. 9 (2024): Journal of Medical Genetics and Clinical Biology
Publisher : PT ANTIS INTERNATIONAL PUBLISHER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61796/jmgcb.v1i9.928

Abstract

Epigenetic modifications is very crucial in managing on/off switch of the genes, and the kind of cell that is being built. The specific goals of this study are to identify the role of histone modifications, DNA methylation, and their interaction with each other on gene expression according to the ChIP-Seq, RNA-Seq, and bisulfite sequencing results. Therefore, in order to offer wide and profound analysis we went to specify the strains for regulatory components, to describe the molecular events, and to contemplate concerning potential clinical utility. Typically, for ChIP-Seq analysis, the number of histone modification peaks varies between 5,000-10,000 per sample, which is the comparison to the input control With regards to the genomic location of these peaks 60-70% of these are in promoter regions, while 20-30% of these are in enhancers. Described variation in RNA-Seq brought about 1000–3000 DE genes per condition; in the compared conditions, the difference generally ranged from 2 to 10 folds. Specifically, 500-1500 of them had different methylation between the control and the patient group with different methylation variations; 20%-60%. Thus, the integration of these datasets demonstrated that there exists significant relationships between histone modification and gene expression level, with the former ment for active modification leading to up-regulation of genes and the latter for repressive modification leading to down- regulation of genes. Besides, the report determined that hypomethylation of promoters of genes led to overexpression whereas hypermethylation of promoters of genes l led to underexpression. The functional enrichment of the genes with the epigenetic changes revealed mostly the cell cycle and signal transduction based on the Gene Ontology.
ROLE OF CYTOTOXIC T LYMPHOCYTE IN ADENOVIRUS INFECTION AL-Abedi, Yasameen Waleed; Alsaeedi, Ali Abdulhussein; Halboti, Alaa Abdalhadi
Journal of Medical Genetics and Clinical Biology Vol. 1 No. 3 (2024): Journal of Medical Genetics and Clinical Biology
Publisher : PT. Antis International Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61796/jmgcb.v1i3.381

Abstract

The successful development of adenovirus vectors for vaccines and gene therapy will require a better understanding of the host immune response. Using the ELISPOT assay to measure IFN-g-secreting CD8þ cells, we identify immunodominant epitopes of the adenovirus hexon and DNA-binding protein in BALB/c and C57BL/6 mice. The T-cell response to the intramuscular administration of adenovirus serotype 5 peaks within a few weeks and gradually declines but is still detectable after 12 weeks. A second administration did not substantially increase the number of reactive T cells. The CD8þ T-cell response was also similar between wild type and E1-deleted adenovirus. When B-cell-deficient mice were injected with adenovirus encoding the gene for secreted alkaline phosphatase, sera phosphatase activity was reduced more quickly in mice pre-exposed to adenovirus. These results add to the evidence that cell-mediated immunity is a substantial barrier to therapeutic adenoviral vectors and provide more quantitative tools to measure cellular immune responses to adenovirus.
UNRAVELING THE ROLE OF EPIGENETIC MODIFICATIONS IN GENE REGULATION A MOLECULAR GENETICS PERSPECTIVE AL-Abedi, Yasameen Waleed; Halboti, Alaa Abdalhadi; Saleh, Ali Abdulhussein
Journal of Medical Genetics and Clinical Biology Vol. 1 No. 9 (2024): Journal of Medical Genetics and Clinical Biology
Publisher : PT. Antis International Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61796/jmgcb.v1i9.928

Abstract

Epigenetic modifications is very crucial in managing on/off switch of the genes, and the kind of cell that is being built. The specific goals of this study are to identify the role of histone modifications, DNA methylation, and their interaction with each other on gene expression according to the ChIP-Seq, RNA-Seq, and bisulfite sequencing results. Therefore, in order to offer wide and profound analysis we went to specify the strains for regulatory components, to describe the molecular events, and to contemplate concerning potential clinical utility. Typically, for ChIP-Seq analysis, the number of histone modification peaks varies between 5,000-10,000 per sample, which is the comparison to the input control With regards to the genomic location of these peaks 60-70% of these are in promoter regions, while 20-30% of these are in enhancers. Described variation in RNA-Seq brought about 1000–3000 DE genes per condition; in the compared conditions, the difference generally ranged from 2 to 10 folds. Specifically, 500-1500 of them had different methylation between the control and the patient group with different methylation variations; 20%-60%. Thus, the integration of these datasets demonstrated that there exists significant relationships between histone modification and gene expression level, with the former ment for active modification leading to up-regulation of genes and the latter for repressive modification leading to down- regulation of genes. Besides, the report determined that hypomethylation of promoters of genes led to overexpression whereas hypermethylation of promoters of genes l led to underexpression. The functional enrichment of the genes with the epigenetic changes revealed mostly the cell cycle and signal transduction based on the Gene Ontology.
Co-Authors AL-Abedi, Yasameen Waleed Alsaeedi, Ali Abdulhussein Saleh, Ali Abdulhussein