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All Journal Journal of Medical Laboratory in Infectious and Degenerative Diseases
Agustin, Rizky Amalia Eka
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Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Public Health

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Test For Antibacterial Activity Of Green Bean Extract (Vigna Radiata L.) Against Bacteria Escherichia Coli Using Well Diffusion Method Agustin, Rizky Amalia Eka
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 1 No. 1 (2023): December
Publisher : LPPM Universitas dr. Soebandi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36858/jmid.v1i1.4

Abstract

Background: Mung beans (Vigna radiata L.) bioactive components that known antibacterial but for their benefits not widely known. This plant includes VIMA 5 mung bean type which the most common variates. Bacteria digestive tract infections Escherichia coliPurpose: to determine antibacterial activity of mung bean extract against bacteria that commonly infect digestive tract,namely Escherichia coli.Methods: Design of this research experimental laboratory using sokhletasi method with ethanol solvent. Sample used ethanol extract mung bean (Vigna radiata L.) concentration dilutions of 10%, 20%, 30% 40%. The positive control used chloramphenicol and negative control DMSO. Inhibition test using the well diffusion method with a diameter of 6 mm. Mung bean compounds contain bioactive components such as flavonoids, tannins, saponins and triterpenoids.Results: The test results show that mung bean seeds have antibacterial activity against Escherichia coli bacteria characterized by the presence of inhibition zones obtained on average for 10% concentration (11.04 mm), 20% concentration (12.04 mm), 30% concentration (12.83 mm), and 40% concentration (13.88 mm) where the whole is classified in the strong category.Conclusions: It can be concluded that the ethanol extract of mung bean seeds (Vigna Radiata L) has antibacterial activity.
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