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All Journal Pharmaciana: Jurnal Kefarmasian
Nurkhasanah, Nurkhasanah
Farmasi UAD
Medicine & Pharmacology

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EFEK FRAKSI ETIL ASETAT EKSTRAK ETANOL AKAR PASAK BUMI (Eurycoma longifolia, Jack) TERHADAP AKTIVITAS FAGOSITOSIS MAKROFAG SECARA IN VITRO Arifah, Aminda Nur; Nurkhasanah, Nurkhasanah
Pharmaciana Vol 4, No 1 (2014): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (284.551 KB) | DOI: 10.12928/pharmaciana.v4i1.392

Abstract

Eurycoma longifolia has been used as a remedy for detoxification, aphrodisiaca, anticancer andimmunistimulant. The quassinoids of pasak bumi root extract, have antioxidant activity andimmunomodulatory activity by increasing IL-12. This study aims to determine the effect of the ethylacetate fraction of ethanolic extract of pasak bumi root on macrophages phagocytic activity in vitro.Macrophages were isolated from peritoneal fluid of male Balb/Cmice, and thenwere cultured for 24hours in 24 well plate. The ethyl actetate fraction ethanolic extract of pasak bumi root withconcentration of 10, 50, and 100 µg/ml, lipopolysaccharides and dimethyl sulfoxide were added, andincubated for 1.5 hours. The phagocytosis tests was carried out by adding latex with density5x104/well and incubated for 1.5 hours at incubator (5% CO2,37oC). Macrophage were then stained byGiemsa. The results showed that the percentage of active phagocytic cells treated with ethyl actetatefraction of ethanolic extract of pasak bumi root of 10, 50 and 100 µg/ml, lipopolysaccharides, anddimethyl sulfoxide were 95.4%, 90%, 85.4%, 83.4%, and 79% respectively. Phagocytic capacity attreatment levels of 10, 50, and 100 µg/ml, lipopolysaccharides,and dimethyl sulfoxide were 469; 439.8; 360.8; 204.6; and 147.6 respectively. The result showed thatreatment of ethyl acetate fractionof ethanolic extract of pasak bumi root can increase the phagocytic activity of macrophages in vitro.
EFEK SITOTOKSIK DAN PEMACUAN APOPTOSIS FRAKSI PETROLEUM ETER EKSTRAK ETANOL DAUN TAPAK LIMAN (Elephantopus scaber Linn) TERHADAP SEL HELA Listyowati, Yeni; Nurkhasanah, Nurkhasanah
Pharmaciana Vol 3, No 2 (2013): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (203.75 KB) | DOI: 10.12928/pharmaciana.v3i2.424

Abstract

Elephantopus scaber Linn. has been reported to have cytotoxic effects on breast cancer cells and thepotential to be developed as anticancer agent. This study aims was to determine the cytotoxic activity andapoptosis induction effect of petroleum ether fractions of ethanolic extract of (Elephantopus scaber Linn) leavesagainst cervical cancer cells (HeLa). Petroleum ether fraction was obtained by dissolving eyhanolic extract inpetroleum ether, and the soluble fraction was as petroleum ether fraction. The method used for cytotoxic activitytest was MTT test. The concentration series used were 2000; 1500; 1000; 800; 400; 200; 100; 50; 25; 12.5; 6.25and 3.125 mg/ml. The IC50 used as cytotoxic parameters. The apoptotic observations was conducted usingacridine orange and ethidium bromide. The study showed the IC50 of petroleum ether fraction of (Elephantopusscaber Linn) ethanolic extract was 185 ug/ml. The study also showed the potency to stimulate apoptosis in HeLacells.
Co-Authors Arifah, Aminda Nur Listyowati, Yeni