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Iesje Lukistyowati
Dosen Fakultas Perikanan dan Kelautan Universitas Riau
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Erythrocyte Profile of Pangasius hypophthalmus Feed with Rhizophora Apiculata Leaf Extract and Maintained in Net Cages Fricilia Zissalwa; Henni Syawal; Iesje Lukistyowati
Jurnal Perikanan dan Kelautan Vol. 25 No. 1 (2020): Februari
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.25.1.70-78

Abstract

Rhizophora apiculata leaf extract has benefits as an herbal supplement that can be added to feed, because it have componds like flavonoid, tannins, saponins, terpenoids, and steroids that can improve the health of jambal fish (Pangasius hypophthalmus). This research was carried out in August to October 2019. The aim of the research was to obtain the best dose of R. apiculata leaf extract added to feed to improve the health of jambal fish as seen from total erythrocytes, hemoglobin levels, hematocrit levels, and erythrocyte morphology. The method was used an experimental method with a Completely Randomized Design (CRD) one factor of four treatment levels consisting of P0: without the addition of extracts; P1: (1.5 mg/kg); P2: (1.7 mg/kg); and P3: (1.9 mg/kg) and repeated three times so that it takes 12 units of the experiment. Maintenance is carried out for 60 days in cages net with a size of 1x1.5x1 m3 out three times, at the beginning of maintenance, 30th day, and 60th day. The best dose was in the P2 treatment (1.7 mg/kg) seen in total erythrocytes 2.57x106 cells/mm3, hemoglobin level 13.13 g/dL, hematocrit value 38.43%, absolute weight growth 38.93 g, survival rate 97,33%, and showed normal erythrocyte morphology, it was supported by water quality during the reasearch such as DO 6.4-6,9 mg/L, pH 6.1-6.8, temperature 28.2 29.50C, and ammonia 0.04-0.08  mg/L. The Blood tests were carried.
Description of Leukocytes Pangasius hypophthalmus which is Fed with Extracts of Mangrove Leaf (Rhizophora apiculata) and Maintained in The Floating Cages Zikra Rahmadona; Henni Syawal; Iesje Lukistyowati
Jurnal Perikanan dan Kelautan Vol. 25 No. 1 (2020): Februari
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.25.1.79-87

Abstract

Mangrove leaves (R.apiculata) contain flavonoid compounds, terpenoids, steroids, saponins, and tannins which function as antibacterial and can improve fish health. This research was carried out in August to October 2019. This research purpose was to know the proper dose of mangrove leaf (R. apiculata) added to the feed to improve fish health as seen from total leukocytes, leukocyte differentiation, and phagocytic activity of P. hypophthalmus. The method in this research was the experimental method using the Completely Randomized Design (CRD) with one factor, four treatment levels and three times replication, so that it takes 12 units of experiments. The treatments were P0 (feed without additional extract R. apiculata), P1, P2, and P3 feed which were given extract R. apiculata at a dose of 1,5 mg/kg, 1,7 mg/kg and 1,9 mg/kg of feed. The fish used is P. hypophthalmus size 8-10 cm with a stocking density of 50 fish/m3. Feeding three times a day at 08.00, 12.00, and 17.00. Blood testing was performed three times, namely the beginning of the 1st, 30th, and 60th day of maintenance. The results showed that the addition of extract (R. apiculata) into the feed had a significant effect on total leukocytes, leukocyte differentiation, phagocytic activity, survival rate and growth of absolute weight of P. hypophthalmus. The best dose is 1,7 mg/kg of feed with total leukocytes 11,17x104 cells/mm3,  lymphocytes 84,33%, neutrophils 6,67%, monocytes 9,00%, phagocytosis activity 35,00%, survival rate 97,33%, and absolute weight growth of 38,93g. 
Co-Authors Fricilia Zissalwa Henni Syawal Zikra Rahmadona