Article Per Year (5 Year)
p-Index From 2020 - 2025
0.408
P-Index
This Author published in this journals
All Journal Caraka Tani: Journal of Sustainable Agriculture
Adyatma Irawan Santosa
Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta
Agriculture, Biological Sciences & Forestry

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Molecular Identification of Three Potyviruses Infecting Allium cepa var. aggregatum and Allium sativum in Central Cultivation Areas of Indonesia Adyatma Irawan Santosa; Muh Amat Nasir; Ali Çelik; Tahir Farooq; Aprilia Sufi Subiastuti
Caraka Tani: Journal of Sustainable Agriculture Vol 39, No 2 (2024): October
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v39i2.86146

Abstract

One hundred and twenty shallot (Allium cepa var. aggregatum) and 22 garlic (Allium sativum) samples were collected from major growing regions and markets to determine the distribution and molecular diversity of 3 potyviruses: leek yellow stripe virus (LYSV), onion yellow dwarf virus (OYDV), and shallot yellow stripe virus (SYSV) in Indonesia. The results of reverse transcription-polymerase chain reaction (RT-PCR) showed that 83% of shallot and all garlic samples were infected by at least 1 virus species. Coat protein (CP) region of 8 Indonesian LYSV, 19 OYDV, and 10 SYSV isolates were sequenced and given accession nos. OR772038-OR772082 in NCBI GenBank. Five isolates were recombinants according to analysis using the Recombination Detection Program (RDP v5.30). The phylogenetic tree deduced that 6 LYSV Indonesian and 2 China imported isolates belong to S-type. All tested OYDV isolates, including the 19 isolates, were clustered separately according to their respective hosts: onion and garlic. The 10 Indonesian SYSV isolates were clustered together in the same group and thus shown to be closely related. All isolates tested in this study were estimated to be still within their respective species demarcation according to percentage identity analysis. This was the most comprehensive molecular study on LYSV, OYDV, and SYSV that may help to find sustainable management strategies according to conditions in Indonesia and contribute to the global knowledge on the genetic diversity of the 3 viruses.
Potato Virus Y and Shallot Latent Virus of Kajoran Horticultural Production Center, Magelang Regency, Indonesia: Molecular Characterization Case Study Adyatma Irawan Santosa; Adenisa Hanifah Irbati; Krisnanda Surya Dharma; Belinda Winona; Reko Saputra Jaya; Annisa Lisa Andriyani; Chilya Qurrota A'yun
Caraka Tani: Journal of Sustainable Agriculture Vol 39, No 1 (2024): April
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v39i1.76737

Abstract

Kajoran Sub-district is a horticultural production center that supplies horticultural commodities for Central Java Province, especially Magelang. However, plant viral diseases of Kajoran have not been studied. This paper aimed to identify plant viral diseases in this horticultural production center. Some samples were taken: three potatoes (Solanum tuberosum) and three shallots (Allium cepa var. aggregatum), then tested respectively with RT-PCR, using two pairs of universal primers to detect Potyvirus and Carlavirus. The result showed two potato samples were infected by Potyvirus (potato virus Y/PVY) and three shallot samples by Carlavirus (shallot latent virus/SLV). GenBank accession no. OR180052 to OR180056 were assigned to the obtained partial sequences of the five isolates. The phylogenetic analysis makes the two new PVY Indonesian isolates in strain N group thus confirmed the presence of the first PVYN in Indonesia. The constructed SLV phylogenetic tree clusters the new three isolates with eight shallot isolates in a group and separated it from isolates from other plant species. The tested PVY isolates shared 95.2 to 100% nucleotide identities among them, while 78.9 to 98.5% identities were estimated among compared SLV isolates. This study expands our understanding of the genetic variation of PVY and SLV in Indonesia, potentially leading us to find the perfect management for the viruses.
Co-Authors Adenisa Hanifah Irbati Ali Çelik Annisa Lisa Andriyani Aprilia Sufi Subiastuti Belinda Winona Chilya Qurrota A'yun Krisnanda Surya Dharma Nasir, Muh Amat Reko Saputra Jaya Tahir Farooq