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All Journal International Journal of Multidisciplinary Research and Literature (IJOMRAL)
Soeryaningsih, Dwie Retna
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Humanities Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Engineering Environmental Science Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Mathematics Physics Social Sciences Other

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OPTIMIZATION OF ISOFLAVONES PRODUCTION ON CALLUS (Pachyrhyzus et al.) THROUGH VARIATIONS IN SOURCES AND AMOUNT OF PLANTING MATERIAL Indarwati, Indarwati; Herawati, Jajuk; Soeryaningsih, Dwie Retna; Widya, Surya Ari; Hassan, Faez M.
International Journal of Multidisciplinary Research and Literature Vol. 3 No. 6 (2024): INTERNATIONAL JOURNAL OF MULTIDISCIPLINARY RESEARCH AND LITERATURE
Publisher : Yayasan Education and Social Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53067/ijomral.v3i6.281

Abstract

In vitro, biotechnology has proven to be a reliable source for producing secondary metabolites. Isoflavones are secondary plant metabolites that have antioxidant and estrogenic properties in the body. Tissue culture technology, which allows the production of plantlets or calluses in small containers, can address challenges in plant metabolite production. To enhance the efficiency of callus production, variations in sources and amount of planting material should be tested in a single planting container. The Bengkoang plant / Jicama (Pachyrhizus erosus) naturally produces isoflavones. This study investigates the impact of variations in sources and amount of planting material on the production of isoflavones in Bengkoang callus using in vitro tissue culture techniques. The research was conducted at the Tissue Culture Laboratory, employing a Completely Randomized Design (CRD) factorial method. Factor I involved the explant source: C1: young leaves; C2: Callus. Factor II involved the number of explants per container: T1: 1; T2: 2; T3: 3. The results show an interaction between source variations and the amount of planting material in anthocyanin production, successfully extracted from the Callus. Planting 3 explants in a container with subculture material from Callus produced the highest 0.16% isoflavones compared to other treatments
Co-Authors Hassan, Faez M. Indarwati Indarwati Jajuk Herawati Widya, Surya Ari