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All Journal Tropical Aquatic and Soil Pollution Industrial and Domestic Waste Management
Okafor, Ugochukwu Chukwuma
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Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Engineering Environmental Science

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Microbiological Quality and Physico-chemical Properties of Bore-Hole Water from Stored Water Tanks in Selected Hostels in Ifite-Awka, Nigeria Okafor, Ugochukwu Chukwuma; Anastasia, Onwugbenu Nneoma; Dike, Umeoduagu Nnamdi
Tropical Aquatic and Soil Pollution Volume 3 - Issue 2 - 2023
Publisher : Tecno Scientifica Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53623/tasp.v3i2.267

Abstract

The microbiological and physicochemical properties of bore-hole waters from water reservoirs in selected hostels in ifite-Awka metropolis were evaluated. Five (5) bore-hole water samples from stored-water tanks were evaluated to ascertain the physicochemical parameters, presence and population of different bacterial and fungal groups. Total heterotrophic bacterial (THC) counts ranged from 1.20x103 cfu/ml to 6.5x103 while the fungal counts spanned from 2.5x103 cfu/ml to 8.9x103 cfu/ml. Bacteria obtained from the borehole waters include Salmonella spp., Escherichia coli and Shigella spp. E. coli was the most prevalent with MPN 380/100 ml of water reported in sample A while Shigella sp. was the least prevalent with MPN 130/100 ml of water reported in samples B and E. Fungal isolates obtained include Aspergillus species, Candida species, Acremonium species and Cladosporium species. E. coli and Aspergillus spp. were predominant than other isolates. The pH ranged from 6.65 to 7.47; hardness ranged from 92 mg/l to 156 mg/l and Iron concentration ranges from 0.267 ppm to 0.378 ppm, phosphate contents ranged from 2.375 to 6.125 while Nitrate contents ranged from 1.071 to 6.214. The presence of these organisms in water meant for municipalities indicates faecal contamination. This calls for improved sanitary conditions of reservoir tanks in these locations and beyond.
Evaluation of the Impact of Crude Oil Contamination on Soil's Physicochemical Characteristics, Micro-flora and Crop Yield Okafor, Ugochukwu Chukwuma
Tropical Aquatic and Soil Pollution Volume 3 - Issue 1 - 2023
Publisher : Tecno Scientifica Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53623/tasp.v3i1.132

Abstract

The effects of crude oil pollution on soil physicochemical properties, microflora, and ecotoxicity were evaluated. Soil samples were contaminated with crude oil, and the effects of contamination on the physicochemical parameters, microflora, and growth index of bean (Phaseolus vulgaris) seeds were studied over a 6-month period. The heterotrophic bacteria isolated from the uncontaminated soil were Micrococcus, Klebsiella, Flavobacterium, Bacillus, Pseudomonas, and Serratia species, and the moulds included microbes such as Aspergillus niger, Fusarium, and Mucor sp. Petroleum contamination increased the pH of the soils to alkaline values while increasing the total nitrogen, organic carbon, and phosphorus contents. Electrical conductivity, nitrogen content, and phosphorus content were significantly reduced after petroleum contamination (p ˂ 0.05). The heavy metal contents of the contaminated soils decreased with increasing remediation time. Zinc, total nitrogen, total organic carbon, and electrical conductivity contents were statistically significantly different among samples throughout the bioremediation period (p ˂ 0.05). The ability of isolates to utilise hydrocarbons was highest for Pseudomonas and Bacillus species and lowest for Klebsiella and Serratia species. After a germination period of 12 days, a germination test showed that the bioattenuated polluted soil improved germination of bean seeds. Bioattenuation methods should be used and improved as a means of remediating petroleum-polluted sites because they are cost-effective and environmentally friendly.
Assessment of Microbial Contaminants and Antibiotic Susceptibility Patterns of Water Tank Samples from Selected Hostels in Ifite-Awka, Nigeria Okafor, Ugochukwu Chukwuma; Eze, Chidera; Iloduba, Ugochukwu Samuel; Nwachineke, Chigozie Emmanuel
Industrial and Domestic Waste Management Volume 5 - Issue 2 - 2025
Publisher : Tecno Scientifica Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53623/idwm.v5i2.727

Abstract

This research assessed the microbial qualities and antibiotic susceptibility of bacterial isolates from water tank samples collected from ten hostels in Ifite-Awka, Nigeria. The samples were cultured on nutrient agar and Sabouraud dextrose agar, and morphological, biochemical, as well as microscopic analyses were carried out. The bacterial isolates included Staphylococcus aureus, Escherichia coli, Salmonella typhi, Bacillus cereus, and Bacillus subtilis. The fungal isolates included Mucor spp., Aspergillus niger, and Penicillium spp. The colonies ranged from 84 to 234 CFU/ml. Across all species, Levofloxacin and Pefloxacin demonstrated the highest activity, indicating broad-spectrum effectiveness. The poorest results were observed with Rifampicin and Gentamicin, as most isolates exhibited complete resistance. The resistance of coliforms such as E. coli and Salmonella typhi to some β-lactams suggested a potential case of antibiotic misuse within the community. Bacillus cereus exhibited the broadest resistance profile. In the fungal analysis, Mucor spp. was the most common (40%), while Aspergillus niger and Penicillium spp. each accounted for 30%. The presence of coliforms in 45% of the samples indicated poor hygiene and placed people’s health in jeopardy. The lack of routine water tank sanitation and consistent bacterial monitoring in the Awka region was particularly concerning given the presence of pathogenic bacteria and toxin-producing fungi. This research highlighted the importance of strict hygiene practices together with efficient cleaning techniques for water tanks in order to reduce microbial contaminants and coliform bacteria.
Co-Authors Anastasia, Onwugbenu Nneoma Dike, Umeoduagu Nnamdi Eze, Chidera Iloduba, Ugochukwu Samuel Nwachineke, Chigozie Emmanuel