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All Journal Journal of Aquaculture and Fish Health Journal of Fish Health
Chowdhury, Sabarna
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Agriculture, Biological Sciences & Forestry

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Evaluation of oxidative stress on Zebrafish under different pH and dissolved oxygen levels using MDA as biomarker: Oxidative stress in Zebrafish Chowdhury, Sabarna; Saikia, Surjya
Journal of Fish Health Vol. 3 No. 2 (2023): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfh.v3i2.3249

Abstract

Oxidative stress on zebrafish (Danio rerio) has been evaluated for pH (four levels namely pH 4.5-5.5, 5.5-6.5, 7.5-8.5 and 8.5-9.5) and Dissolved Oxygen (DO 20-30%, 40-50% and 60-70%) in terms of oxygen saturation. Temporally, the exposure for pH levels were maintained from as 1h, 2h, 3h and 4h and for DO, it was (4h, 8h, 12h and 16h. The malondialdehyde (MDA) was used as biomarker of oxidative stress. It was observed that out of all tissues (skeletal muscle, liver, gill and brain) analyzed, zebrafish showed highest stress response in skeletal muscle against both environmental ambiances. In case of pH levels, significant increase in MDA level in skeletal muscle was recorded at pH 4.5-5.5, and the MDA level of skeletal muscle at 2h showed significant increase at this level of pH. Whereas, at DO 20-30% saturation level, skeletal muscle tissue significantly responded at this level of DO saturation and showed the maximum MDA level compared to the other tissues. On analysis for the duration of exposure, it was observed that skeletal muscle of zebrafish showed highest levels of MDA at DO 40-50% O2 saturation for 12h. In conclusion, it has become evident that zebrafish may undergo oxidative stress at pH 4.5-5.5 when exposed for 2hours, similarly, for DO it is 20-30% saturation for 12 hour exposure.
Effect of two aquatic ambient factors (pH and Dissolved Oxygen) on antioxidant generation in skeletal muscle of zebrafish (Danio rerio, Hamilton 1822) Chowdhury, Sabarna; Saikia, Surjya
Journal of Aquaculture and Fish Health Vol. 13 No. 2 (2024): JAFH Vol. 13 No. 2 June 2024
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v13i2.49937

Abstract

Ambient factors in aquatic body are often responsible for oxidative stress in fish. Zebrafish has been frequently targeted for study to understand such hypoxic effects. In this study, two ambient factors, viz. DO saturation (20-30%, 40-50%, 60-70% and, 80% and above) and pH (4.5-5.5, 5.5-6.5, 6.5-7.5, 7.5-8.5 and 8.5-9.5) were experimented for generation of antioxidant (Catalase, SOD and Glutathione) in the skeletal muscle of zebrafish. The oxidative stress was marked with the levels of MDA in the skeletal muscle. These conditions were tested against the length of treatment period (in hr) from 4hr to 16 hr for each DO saturation level and 1hr to 4 hr for each pH level. The analysis of data shows that, for DO saturation, the skeletal muscle of zebrafish suffered maximum at 12 hr of treatment period having highest level of MDA against 20-30% of saturation level. Similarly, for pH, this period was 2hr and the treatment level was pH 4.5-5.5. Although alkaline ambiance (pH 8.5-9.5) also exerted strong oxidative stress at 2 hr, but it was significantly low in terms of generation of MDA.  In all cases, the antioxidant levels spiked after post maximum generation period i.e. 16 hr for DO saturation and 2 hr for pH level. This indicates a time point wherefrom the fish undergoes oxidative stress and initiation of a counter mechanism during the post oxidative stress period in these two ambient conditions. A two-way ANOVA has explained that the effect of treatment period and the level of ambient factors significantly contributed to the antioxidant generation in zebrafish. The outcome of this work will directly help in management of fish culture when the aquatic body turns to hypoxic or acidic in nature.
Evaluation of oxidative stress on Zebrafish under different pH and dissolved oxygen levels using MDA as biomarker: Oxidative stress in Zebrafish Chowdhury, Sabarna; Saikia, Surjya
Journal of Fish Health Vol. 3 No. 2 (2023): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfh.v3i2.3249

Abstract

Oxidative stress on zebrafish (Danio rerio) has been evaluated for pH (four levels namely pH 4.5-5.5, 5.5-6.5, 7.5-8.5 and 8.5-9.5) and Dissolved Oxygen (DO 20-30%, 40-50% and 60-70%) in terms of oxygen saturation. Temporally, the exposure for pH levels were maintained from as 1h, 2h, 3h and 4h and for DO, it was (4h, 8h, 12h and 16h. The malondialdehyde (MDA) was used as biomarker of oxidative stress. It was observed that out of all tissues (skeletal muscle, liver, gill and brain) analyzed, zebrafish showed highest stress response in skeletal muscle against both environmental ambiances. In case of pH levels, significant increase in MDA level in skeletal muscle was recorded at pH 4.5-5.5, and the MDA level of skeletal muscle at 2h showed significant increase at this level of pH. Whereas, at DO 20-30% saturation level, skeletal muscle tissue significantly responded at this level of DO saturation and showed the maximum MDA level compared to the other tissues. On analysis for the duration of exposure, it was observed that skeletal muscle of zebrafish showed highest levels of MDA at DO 40-50% O2 saturation for 12h. In conclusion, it has become evident that zebrafish may undergo oxidative stress at pH 4.5-5.5 when exposed for 2hours, similarly, for DO it is 20-30% saturation for 12 hour exposure.
Co-Authors Saikia, Surjya