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HEMATOLOGY OF Osphronemus goramy FED CONTAINING FERMENTED TURI (Sesbania grandiflora) LEAF MEAL and CHALLENGED WITH Aeromonas hydrophila Wahyono, Hendri; Luksityowati, Iesje; Riauwaty, Morina
Asian Journal of Aquatic Sciences Vol. 6 No. 3 (2023): December
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Turi leaf (Sesbania grandiflora) is a natural ingredient with antibacterial compounds. This research was conducted from April to June 2022 at the Fish Nutrition Laboratory and the Laboratory of Parasites and Fish Diseases, Faculty of Fisheries and Marine, Universitas Riau. This study aimed to obtain the best dose of fermented turi leaves in feed seen from hematology before and after the challenge test with A.hydrophila. The method used in this study is an experimental method using a one-factor Completely Randomised Design (CRD) with five treatment levels, namely Kn: (no treatment), Kp: (no cure and infected with A.hydrophila), P1: (fermented feed containing 15% turi leaves, 85% soy flour), P2: (30% turi leaves, 70% soy flour), and P3: (45% turi leaves, 55% soy flour). Fish were reared for 46 days. The challenge test was conducted on day 32 with A.hydrophila bacteria at a density of 108 CFU/mL, as much as 0.1 mL/head. The results showed that fermented feed contained 45% turi leaves and 55% soybeans (P3). Wheat flour obtained total erythrocytes 2.51x106 cells/mm3, hematocrit 40.33%, hemoglobin 10.40 g/dL, total leukocytes 10.13x104 cells/mm3, phagocytosis activity 21.13%, survival rate 90%, and absolute weight growth 9.52g. Water quality parameters were temperature ranging from 27-300C, pH 5.9-6.2, DO 5.5-5.9 mg/L, and NH3 0.015-0.017 m g/L. Based on the results of the study, it can be concluded that there is an effect of adding fermented turi leaves to feed on the hematology of gouramy before and after the A.hydrophila bacterial challenge test has a significant impact.

THE USE OF Kaempferia galanga AND Curcuma xanthorrhiza FERMENTED TO IMPROVE THE IMMUNITY OF Oreochromis niloticus AGAINST Streptococcosis Khairia, Khairia; Syawal, Henni; Luksityowati, Iesje; Riauwaty, Morina
Asian Journal of Aquatic Sciences Vol. 6 No. 3 (2023): December
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Streptococcus agalactiae is a bacterium that causes streptococcosis, which attacks tilapia. This research was conducted from March to June 2022 at the Laboratory of Parasites and Fish Diseases, Faculty of Fisheries and Marine, University of Riau. This study aims to understand the best dose of fermented Kaempferia galanga and Curcuma xanthorrhiza, which are added to the feed to increase Oreochromis niloticus immunity. The method used was experimental with a completely randomized design (CRD) with five treatment levels and three replications. The treatments applied were Kn: Negative control (no treatment used), Kp: positive control (no treatment used and infected with S.agalactiae), P1 (100 mL/kg feed), P2 (125 mL/kg feed), P3 (100 mL/kg feed) and infected with S. agalactiae. The fish were contaminated on the 32nd day at a density of bacteria 108 CFU/mL of 0.1 mL/fish intramuscularly, and then the fish were reared up to the 46th day. The results showed that the fermented K.galanga and C.xanthorrhiza affected the haematology of O.niloticus. The best dose was in P3 with a dose of 150 ml/kg of feed which showed total erythrocytes of 2.01 x106 cells/mm3, hematocrit value of 27.33%, a hemoglobin level of 7.93 g/dL, total leukocytes of 6.59 x104 cells/mm3, lymphocytes 77.33%, monocytes 14.00%, neutrophils 8.67%, growth with a weight of 34.06 g, a length of 7.5 cm and a survival rate of 93.33%. Based on the data obtained, it can be concluded that fermented K.galanga and C.xanthorrhiza can increase the immunity of O.niloticus against Streptococcosis disease.

HEMATOLOGY OF Osphronemus goramy FED CONTAINING FERMENTED TURI (Sesbania grandiflora) LEAF MEAL and CHALLENGED WITH Aeromonas hydrophila Wahyono, Hendri; Luksityowati, Iesje; Riauwaty, Morina
Asian Journal of Aquatic Sciences Vol. 6 No. 3 (2023): December
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Turi leaf (Sesbania grandiflora) is a natural ingredient with antibacterial compounds. This research was conducted from April to June 2022 at the Fish Nutrition Laboratory and the Laboratory of Parasites and Fish Diseases, Faculty of Fisheries and Marine, Universitas Riau. This study aimed to obtain the best dose of fermented turi leaves in feed seen from hematology before and after the challenge test with A.hydrophila. The method used in this study is an experimental method using a one-factor Completely Randomised Design (CRD) with five treatment levels, namely Kn: (no treatment), Kp: (no cure and infected with A.hydrophila), P1: (fermented feed containing 15% turi leaves, 85% soy flour), P2: (30% turi leaves, 70% soy flour), and P3: (45% turi leaves, 55% soy flour). Fish were reared for 46 days. The challenge test was conducted on day 32 with A.hydrophila bacteria at a density of 108 CFU/mL, as much as 0.1 mL/head. The results showed that fermented feed contained 45% turi leaves and 55% soybeans (P3). Wheat flour obtained total erythrocytes 2.51x106 cells/mm3, hematocrit 40.33%, hemoglobin 10.40 g/dL, total leukocytes 10.13x104 cells/mm3, phagocytosis activity 21.13%, survival rate 90%, and absolute weight growth 9.52g. Water quality parameters were temperature ranging from 27-300C, pH 5.9-6.2, DO 5.5-5.9 mg/L, and NH3 0.015-0.017 m g/L. Based on the results of the study, it can be concluded that there is an effect of adding fermented turi leaves to feed on the hematology of gouramy before and after the A.hydrophila bacterial challenge test has a significant impact.

THE USE OF Kaempferia galanga AND Curcuma xanthorrhiza FERMENTED TO IMPROVE THE IMMUNITY OF Oreochromis niloticus AGAINST Streptococcosis Khairia, Khairia; Syawal, Henni; Luksityowati, Iesje; Riauwaty, Morina
Asian Journal of Aquatic Sciences Vol. 6 No. 3 (2023): December
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Streptococcus agalactiae is a bacterium that causes streptococcosis, which attacks tilapia. This research was conducted from March to June 2022 at the Laboratory of Parasites and Fish Diseases, Faculty of Fisheries and Marine, University of Riau. This study aims to understand the best dose of fermented Kaempferia galanga and Curcuma xanthorrhiza, which are added to the feed to increase Oreochromis niloticus immunity. The method used was experimental with a completely randomized design (CRD) with five treatment levels and three replications. The treatments applied were Kn: Negative control (no treatment used), Kp: positive control (no treatment used and infected with S.agalactiae), P1 (100 mL/kg feed), P2 (125 mL/kg feed), P3 (100 mL/kg feed) and infected with S. agalactiae. The fish were contaminated on the 32nd day at a density of bacteria 108 CFU/mL of 0.1 mL/fish intramuscularly, and then the fish were reared up to the 46th day. The results showed that the fermented K.galanga and C.xanthorrhiza affected the haematology of O.niloticus. The best dose was in P3 with a dose of 150 ml/kg of feed which showed total erythrocytes of 2.01 x106 cells/mm3, hematocrit value of 27.33%, a hemoglobin level of 7.93 g/dL, total leukocytes of 6.59 x104 cells/mm3, lymphocytes 77.33%, monocytes 14.00%, neutrophils 8.67%, growth with a weight of 34.06 g, a length of 7.5 cm and a survival rate of 93.33%. Based on the data obtained, it can be concluded that fermented K.galanga and C.xanthorrhiza can increase the immunity of O.niloticus against Streptococcosis disease

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