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All Journal Acta Biochimica Indonesiana Jurnal Jamu Indonesia Indonesian Journal of Jamu
Purwaningsih, Erni
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Medicine & Pharmacology Other

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Total Phenolic Content in Black Rice (Oryza sativa L. indica) Bran Ethanolic Extract from Two Different Regions in Java, Indonesia Vardhani, Afifah; Anggraini, Nadia Bunga; Jufri, Mahdi; Purwaningsih, Erni; Perdanastuti, Hidayah Sunar
Jurnal Jamu Indonesia Vol. 9 No. 1 (2024): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v9i1.293

Abstract

Rice bran, a rice by-product yielded by the milling process, has recently been recognized as a potential source of natural active constituents. Black rice bran has the highest antioxidant capacity compared to white and brown rice bran. It is correlated with the contribution of phenolic compounds in rice bran. The purpose of this study was to determine the total phenolic content in black rice (Oryza sativa L. indica) bran ethanolic extract from two different regions in Java, Indonesia, using Folin-Ciocalteu Reagent (FCR). Black rice bran samples used in this study were planted in Ciletuh Geopark Sukabumi, West Java (BRBE1), and Karanganyar Regency, Central Java (BRBE2). The parameters observed were total phenolic content, and the result was statistically tested with an unpaired t-test by GraphPad Prism 8.3.0 application. The samples contained 175.48 mg GAE/g extract in BRBE1 and 174.39 mg GAE/g extract in BRBE2. There was no significant difference based on these results. It can be concluded that both samples contained phenolic compounds and statistically, there was no significant difference.
Total Phenolic Content in Black Rice (Oryza sativa L. indica) Bran Ethanolic Extract from Two Different Regions in Java, Indonesia Vardhani, Afifah; Anggraini, Nadia Bunga; Jufri, Mahdi; Purwaningsih, Erni; Perdanastuti, Hidayah Sunar
Jurnal Jamu Indonesia Vol. 9 No. 1 (2024): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v9i1.293

Abstract

Rice bran, a rice by-product yielded by the milling process, has recently been recognized as a potential source of natural active constituents. Black rice bran has the highest antioxidant capacity compared to white and brown rice bran. It is correlated with the contribution of phenolic compounds in rice bran. The purpose of this study was to determine the total phenolic content in black rice (Oryza sativa L. indica) bran ethanolic extract from two different regions in Java, Indonesia, using Folin-Ciocalteu Reagent (FCR). Black rice bran samples used in this study were planted in Ciletuh Geopark Sukabumi, West Java (BRBE1), and Karanganyar Regency, Central Java (BRBE2). The parameters observed were total phenolic content, and the result was statistically tested with an unpaired t-test by GraphPad Prism 8.3.0 application. The samples contained 175.48 mg GAE/g extract in BRBE1 and 174.39 mg GAE/g extract in BRBE2. There was no significant difference based on these results. It can be concluded that both samples contained phenolic compounds and statistically, there was no significant difference.
Comparing the effect of Centella asiatica L. and Acalypha indica L. treatment to carbonyl and glutathione level in the brains of old rats Mudjihartini, Ninik; Paramita, Reni; Siregar, Astrid Mariam Khairani; Filzadiyanti, Estiana; Sarsanti, Pungguri Ayu Nega; Purwaningsih, Erni
Acta Biochimica Indonesiana Vol. 5 No. 1 (2022): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.79

Abstract

Background: Free radicals in excessive concentrations damages cells and accelerate the aging process. Antioxidants found in Centella asiatica (CA) and Acalypha indica (AI) have the potential to prevent oxidative cellular damage. Objective: This study aimed to determine the effect of CA and AI on carbonyl and glutathione levels in the brain of older rats. Methods: 18-month age rats were treated using either AI, CA, or vitamin E. In addition, 18-month age and 2-month age untreated rats were used as a negative control. The brain carbonyl and glutathione levels were measured by Agustyanak and the Elmann method, respectively. Results: Treatment with CA significantly decreased brain carbonyl levels (2.87 nmol/mL) than the control rats (4.54 nmol/mL). Furthermore, treating AI did not reduce the brain carbonyl and GSH levels in aged brain rats. Conclusion: Centella asiatica can reduce the protein destruction that occurs with increasing age.
Co-Authors Anggraini, Nadia Bunga Filzadiyanti, Estiana Mahdi Jufri Ninik Mudjihartini Perdanastuti, Hidayah Sunar Reni Paramita Sarsanti, Pungguri Ayu Nega Siregar, Astrid Mariam Khairani Vardhani, Afifah