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All Journal EKSAKTA: Journal of Sciences and Data Analysis
Josua Kristinao Hilmanto
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Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Materials Science & Nanotechnology

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The Isolation and Identification of Thiamine-Binding Protein from Black Glutinous Rice Bran (Oryza Sativa var. Glutinosa) Gunarti, Dwirini; Josua Kristinao Hilmanto
EKSAKTA: Journal of Sciences and Data Analysis VOLUME 5, ISSUE 2, October 2024
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/EKSAKTA.vol5.iss2.art6

Abstract

Thiamine is a crucial cofactor in energy metabolism. Deficiency of this vitamin can cause disorders in the nervous and cardiovascular systems. Conventional methods for examining thiamine levels in the body are expensive. One solution is using a technique similar to ELISA, namely ELPLA, which uses thiamine-binding protein as an antibody substitute. This study aims to investigate the presence of thiamine-binding protein in black glutinous rice bran (Oryza Sativa var. Glutinosa). The methods used include salting out, dialysis, and equilibrium dialysis. The results show the presence of thiamine-binding protein in black glutinous rice bran, evidenced by equilibrium dialysis.
Co-Authors Gunarti, Dwirini