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All Journal Journal of Food and Culinary Narra J
Hanifah, Wafiq
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Economics, Econometrics & Finance Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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In silico analysis of Arbacia lixula-derived peptides and plasmid construction for recombinant anti-aging therapies Yenny, Satya W.; Jamsari, Jamsari; Hazmi, Auliya A.; Cuandra, Kevin N.; Hanifah, Wafiq; Yahono, Angela S.; Wahyudi, Dhyani P.; Buana, Gherriandi R.; Rahman, Awalil RK.; Maharani, Annisa D.; Firjatullah, Muhammad F.; Maulana, Rafi; Prayogi, Norbertus M.; Tristan, Christopher D.
Narra J Vol. 4 No. 3 (2024): December 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i3.1283

Abstract

Skin aging is one of the degenerative processes influenced by tyrosinase, elastase, collagenase, hyaluronidase, and matrix metalloproteinase-9 (MMP9) activity. One promising avenue for discovering antiaging therapeutics is the peptides from the Arbacia lixula spine. The aim of this study was to explore the potential of peptides from A. lixula spine as a multitarget inhibitor for recombinant antiaging therapies through in silico approaches. The crystal structure of peptides previously identified in A. lixula spine was visualized using the UCSF Chimera. The protein data bank (PDB) database was used to obtain the crystal structures of protein targets. The webservers Innovagen, AllerTop, and ToxinPred were utilized to predict the peptide's water solubility, toxicity, and allergenicity. MOE application was used to prepare all ligands and proteins, molecular docking, and visualization. Molecular dynamics simulations were carried out on the protein-ligand complexes on Yasara Dynamics application. The Benchling website was used to perform virtual electrophoresis and reconstruct the recombinant plasmid (Psb1c3). Based on the molecular docking results, peptide REGSPDLLE has the potential as a multitarget inhibitor of tyrosinase (-9.07 kcal/mol), hyaluronidase (-10.57 kcal/mol), elastase (-9.32 kcal/mol), collagenase (-10.57 kcal/mol), and MMP9 (-10.43 kcal/mol). Peptide REGSPDLLE was selected due to its strong binding affinity on the active site of each target protein and exhibits non-toxic, non-allergenic, and good water-soluble as indicated by Support Vector Machine score <0. Molecular dynamics simulations confirmed stable interactions with receptor proteins. Peptide REGSPDLLE was successfully inserted into the recombinant pSB1C3 plasmid, confirmed by virtual electrophoresis with bands at ~2000 bp and ~150 bp. Further in vitro and in vivo studies are necessary to verify the anti-aging efficacy of peptide REGSPDLLE.
Potential Effects of Javanese Chili (Piper retrofractum Vahl.) Kombucha to Reduce Hyperglycemic and Maintain Spatial Memory: In Vitro and In Vivo Studies Suyanti, Eri Dwi; Tsani, Salma Mutiara; Hanifah, Wafiq; Nastiti, Fithratun; Maatita, Marcellino; Farmawati, Arta
Journal of Food and Culinary Vol. 8 No. 1 [Juni 2025]
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jfc.v8i1.13598

Abstract

Type 2 Diabetes Mellitus (T2DM) and Alzheimer's Disease (AD) are diseases which incidence and prevalence increase with age. Diabetic patients have 50-75% higher risk of developing AD compared to patients without diabetes. This study aimed to determine the potential effect of Javanese chili kombucha to reduce hyperglycemia and to act as a neuroprotective in diabetes patients with a risk of AD complications through in vitro and in vivo studies. The experiment carried out was fermentation of Javanese chili kombucha for 3, 6, 9, 12 and 15 days. Organoleptic tests, pH measurements, total titratable acidity (TTA), antioxidant tests and α-amylase inhibition tests were conducted to determine the best duration for kombucha fermentation. Phytochemical screening and in vivo tests using 5 groups of rats included measurements of fasting blood glucose (FBG), malondialdehyde (MDA) assay and antidementia (T-maze). The 9th day fermentation preparation was the best preparation, with organoleptic test results of brown color, sour smell with a pH of 3.43 (safe), an antioxidant IC50 value of 10.5 (very strong), an α-amylase inhibition IC50 value of 117.14 (moderate) and TTA 1.44 (normal). Phytochemical screening results show that Javanese chili kombucha contains alkaloids, flavonoids, terpenoids/steroids, saponins, and phenolics. Based on in vivo tests, Javanese chili kombucha can reduce FBG levels and can maintain the spatial memory of experimental animals with a statistically significant T-maze duration time between groups. The MDA level test showed oxidative stress results that were not significantly different between groups.
Co-Authors Arta Farmawati Buana, Gherriandi R. Cuandra, Kevin N. Firjatullah, Muhammad F. Hazmi, Auliya A. Jamsari Jamsari Maatita, Marcellino Maharani, Annisa D. Maulana, Rafi Nastiti, Fithratun Prayogi, Norbertus M. Rahman, Awalil RK. Suyanti, Eri Dwi Tristan, Christopher D. Tsani, Salma Mutiara Wahyudi, Dhyani P. Yahono, Angela S. Yenny, Satya W.