Article Per Year (5 Year)
p-Index From 2021 - 2026
0
P-Index
This Author published in this journals
All Journal BERITA BIOLOGI
Djajanegara, Ira N
Research Center for Biology-Indonesian Institute of Sciences
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
KONDISI OPTIMUM FUSI PROTOPLAS ANTARA JAMUR TIRAM PUTIH {Peurotus floridae) DAN JAMUR TIRAM COKLAT {Pleurotus cystidiosusy Djajanegara, Ira N; El-khobar, Korri
BERITA BIOLOGI Vol 9, No 5 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1017.932 KB) | DOI: 10.14203/beritabiologi.v9i5.1997

Abstract

Genetic crossing of white oyster mushroom to introduce longer storage life trait can only be done within individuals in this particular species. However, longer storage life trait is possessed by brown oyster mushroom (Pleurotus cystidiosus) which is other species within this genus. Thefeore, protoplast fusion between white oyster mushroom (Peurotus floridae) and brown oyster mushroom (Pleurotus cystidiosus) was conducted to hopefully obtain an oyster mushroom strain that has higher production and longer storage life. Protoplast fusion was done by isolating protoplast from 5-days old monokaryotic mycelia grown in PDB. As much as 3.15 x 10 protoplasts/ml were harvested using mixture of cellulase Onozuka R-10 (1%) and macerozyme R-10 (1%) from brown oyster mushroom with 80.61% viability. Similarly, 3.71 x 10 protoplasts/ml were harvested using lysing enzyme (2%)from brown oyster mushroom with 83.68% viability. Protoplast fusion were conducted using 0% (control), 30%, 40% and 50% of PEG6000. Fusion periods were done at 10, 20 and 30 minutes. The candidate fusants were then screened using MRM (minimum regeneration media) media. Based on this experiment, the optimum conditions for protoplast fusion is 10 minutes incubation using 40% PEG6000 that yielded 121 colonies grown on MRM media as candidate fusants.
Co-Authors El-khobar, Korri