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Supriyati, Dyah
Research Center for Biology-Indonesian Institute of Sciences

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Stappia aggregata Gl DAN Alteromonas sp.G2 BAKTERIPENDEGRADASI PHENANTRENE YANG DIISOLASIDARI LINGKUNGAN LAUT Supriyati, Dyah
BERITA BIOLOGI Vol 9, No 6 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (504.144 KB) | DOI: 10.14203/beritabiologi.v9i6.842

Abstract

Stappia aggregata Gl and Alteromonas sp. G2 are marine bacteria isolated from Kepulauan Seribu waters, Jakarta. These bacteria were isolated using ONR7a media, and forming clear zone surrounding grown colonies after 48 hours, and able to grow on phenantrene as the sole carbon sources. After 4 hours incubation phnenantrene was degraded, and almost 12 hours about 60% of phenantrene was converted. The two strains performed similar biodegradation pattern, but different in growth characteristic.Bacteria Stappia aggregata Gl and Alteromonas sp. G2 grew optimum at 30°C, pH 8 and 3% salinity.
ABSORBSI GLUKOSA DAN SUKROSA SEBAGAI SUMBER KARBON UTAMA OLEH KOMUNITAS MPG PADA KONDISI ANAEROBIK-AEROBIK Supriyati, Dyah
BERITA BIOLOGI Vol 9, No 5 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (782.598 KB) | DOI: 10.14203/beritabiologi.v9i5.1988

Abstract

To study the role of substrate on the establishment of glycogen accumulating organism (MPG), activated sludge originated from anaerobic-aerobic process was implemented on waste water treatment plant which was acclimated with glucose and sucrose.Both susbtrates were effectively utilized by microbial communities during anaerobic condition. Those absorbed substances were converted to glycogen.Glycogen was also used as energy generating process as indicated by a decrease of glycogen. The dominancy of glycogen accumulating organism suppresses the polyphosphate accumulating organism as indicated by no significant release of orthosphosphate during anaerobic condition.
KOMUNITAS MIKROBA PENGAKUMULASI GLIKOGEN Supriyati, Dyah; Rahayu, Rita Dwi; Imamuddin, Hartati
BERITA BIOLOGI Vol 8, No 5 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i5.1896

Abstract

Activated sludge originated from anaerobic-aerobic process waste water treatment plan was acclimated with glucose and acetate.The experiment was conducted in 1 L working volume of sequential batch reactor (SBR) that was adjusted to 0, 25 kg m day.Glucose was effectively utilized by microbial community in anaerobic condition, and glycogen synthesis was occurred in aerobic condition.Suppression of polyphosphate accumulating organism was caused by the domination of glycogen accumulating organism and the high of nitrate production. In order to accelerate the community polyphosphate accumulating organisms then to the competitor of these communities must be pressured.
PEMANFAATAN BERBAGAI SENYAWA NITRIL DAN PRODUK DEGRADASINYA SEBAGAI SUBSTRAT UNTUK PERTUMBUHAN ISOLAT BAKTERI TP Supriyati, Dyah; Sunarko, Bambang
BERITA BIOLOGI Vol 5, No 4 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (439.201 KB) | DOI: 10.14203/beritabiologi.v5i4.1129

Abstract

Our experiments showed that bacterial isolate TP was able to grow on acetonitrile, butyronitrile and propionitrile as sole sources of carbon, energy and nitrogen, but not on acrylonitrile dan benzonitrile. Besides on nitriles, isolate TP could grow on acetamide,propionamide, benzamide and nicotinamide, but not on acrylamide. However, none of the tested carboxylic acids could be used as growth subtrate for bacterial isolate TP. The best growth substrates of isolate TP were butyronitrile (CH3-CH2-CH2-CN) and propionamide (CH3-CH2-CONH2). When isolate TP grew on butyronitrile, the highest biomass concentration, the doubling-time (td), and the specific growth rate1(n) were 8.99 gram cell dry weight/liter, 4.8 h and 0.144 h , and when grew on propionamide were 4.57 gram cell dry weight/liter, 5.7 h and10.122 h", respectively.