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All Journal Biotropika Acta Solum
Sahana, Laudy Arrisa Arumsari
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Isolation and Identification of Mercury-Resistant Fungi as Bioremediation Agents from Gold Mining Sahana, Laudy Arrisa Arumsari; Mohamad , Yani; Rohani Cinta Badia Br , Ginting
Biotropika: Journal of Tropical Biology Vol. 12 No. 2 (2024)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2024.012.02.01

Abstract

To overcome the pollution problem due to the accumulation of heavy metal Hg, a clean-up or remediation effort is needed from the former landfill for hazardous and toxic waste.  In this research, bioremediation experiments will be conducted by inoculating fungi culture that is already known to have the potential to absorb heavy metals Hg. Samples of soil contaminated with heavy metals Hg taken from the tailings of gold mining owned by the people in Kertajaya Village, Simpenan District, Sukabumi Regency. The stages of this study consisted of isolation of fungi, purification and morphology of fungi isolate, screening of fungi isolate, qualitative screening of fungi for ligninolytic activity (laccase), and molecular identification of the isolated fungi. The results of the screening showed that three isolates of potential fungi coded M.2, M.10, and M.13 were able to grow with a mercury concentration of 350 ppm, while one isolate coded R4.28 reached a concentration of 1000 ppm. In addition, isolate M.2 was selected as the best strain compared to isolates M.10, M.13, and R4.28 for the qualitative test of laccase production in ligninolytic enzymes. Based on the molecular analysis of the 18S rRNA gene, M.2 isolates were similar to Trichoderma yunnanense, which was strengthened by a similarity value of 99.66%. Isolate R4.28 was similar to Penicillium soli, which was strengthened by a similarity value of 99.32%.
Unveiling the BPF9 Isolate as a Potential of Phosphate-Solubilizing Bacteria Through In Vitro Characterization Putri, Devanda Ayu Lidya Permata; Putra, Guruh Mayka; Sahana, Laudy Arrisa Arumsari; Nirmalasari, Intan
Acta Solum Vol. 3 No. 3 (2025): Juli 2025
Publisher : Department of Soil, Faculty of Agriculture, Lambung Mangkurat University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/actasolum.v3i3.3329

Abstract

The increasing demand for food has driven agricultural intensification, particularly through the use of phosphate fertilizers. However, phosphorus uptake efficiency by plants remains low due to its predominance in insoluble forms in the soil. One promising alternative is the use of phosphate-solubilizing bacteria (PSB). This study aimed to evaluate the solubilization potential of the BPF9 isolate through qualitative and quantitative assays, as well as growth curve profiling. The research was conducted at the Soil and Environmental Biotechnology Laboratory, IPB University. Qualitative phosphate solubilization was assessed on Pikovskaya agar by measuring halo zone formation to calculate the solubilization index (SI). Quantitative analysis was carried out using spectrophotometry at 660 nm. The bacterial growth curve was analyzed using the haemocytometer method. Results showed that BPF9 had a solubilization index of 2.0 (moderate category) and was able to solubilize phosphate up to 128.5 ppm, which is considered highly efficient. The growth curve indicated that BPF9 entered the exponential phase at 26 hours and reached its maximum population at 42 hours. These findings indicate that BPF9 possesses strong phosphate-solubilizing capability and stable growth characteristics, making it a promising candidate for biofertilizer development.
Co-Authors Devanda Ayu Lidya Permata Putri INTAN NIRMALASARI Mohamad , Yani Putra, Guruh Mayka Rohani Cinta Badia Br , Ginting