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All Journal BERKALA SAINSTEK Indonesian Chimica Letters
Anida, Choirun Nisa
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Mathematics Physics

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Methyl Red Based Test Strip for Identification of Formalin Compounds in Fish Samples Haji, Lina Sun; Anida, Choirun Nisa; Aprilianti, Caleysda; Fauziyyah, Himayah Izmi; Pitaloka, Diah Ayu; Zulfikar, Zulfikar
BERKALA SAINSTEK Vol 12 No 3 (2024)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v12i3.52156

Abstract

Methyl red based test strips were introduced a simple method for formalin identification. The test strip was designed based on the feasibility of the formalin oxidation reaction using hydrogen peroxide in an alkaline medium and using the methyl red indicator which is detected at a wavelength of 526 nm, with a concentration range from 1.0 - 10.0 ppm. The test strip was designed by immobilizing methyl red and NaOH in a Whatman paper matrix number 40 for 40 minutes and it has been evaluated by adding formalin samples and oxidator to the surface of the test strip. Optimum measurement conditions were obtained at 0.05 mL of 0.06M methyl red, 0.2 mL of 0.001M NaOH and 0.25 mL of 2% H2O2. The test strip has a good performance to identify formalin in the concentration range of 1.0 - 7.5 ppm which is monitored by a color change from yellow to red. The feasibility of using the test strip is also carried out by analyzing real samples through a recovery test. Test strips still can be used to identify formalin until 30 days when it stored at room temperature, and 2 days when it stored in the refrigerator.
Development of Arduino Uno Based Multispectra Colorimeter For Formaldehyde Determination Anida, Choirun Nisa; Zulfikar, Zulfikar; Siswoyo, Siswoyo; Asnawati, Asnawati; Reza, Muhammad
Indonesian Chimica Letters Vol. 4 No. 1 (2025)
Publisher : Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/icl.v4i1.5693

Abstract

Formalin is a hazardous substance that is often misused as a food preservative, so routine formalin analysis is very necessary for public safety. Formalin analysis generally uses spectrophotometry and also a simple colorimeter. Currently, many Arduino-based colorimeters are being studied and development with RGB (Red, Green, Blue) LED (Light Emitting Diode) light sources. This research is also in this field with a focus on the use of multi-spectral RGB LED lights with 16 colors that are already commercially available. The multispectral colorimeter is designed by integrating an LED light source, a photodiode module as a detector, and controlled by an Arduino Uno R3. The LED light module circuit, cuvette and detector photodiode are then arranged and packaged in an X8 project box. Optimization of the circuit is studied including the distance between the LED lamp and the cuvette and the photodiode with the cuvette. A feasibility study of the use of multispectral RGB LEDs is also carried out by looking at the stability of the response of each color. The performance test of the multispectral colorimeter is carried out by analyzing formaldehyde oxidized with hydrogen peroxide using methyl red as an indicator under the alkaline condition. The results showed no significant difference when viewed from the linearity, Limit of Detection, Limit of Quantitation, sensitivity, and precision values calculated from the calibration curve. The difference in linearity values was 0.0013, Limit of Detection = 0.17, Limit of Quantitation = 0.57, sensitivity = 0.0001, and precision (SD = 0.001 and %RSD = 0.11).
Co-Authors Aprilianti, Caleysda Asnawati Asnawati Fauziyyah, Himayah Izmi Haji, Lina Sun Muhammad Reza Pitaloka, Diah Ayu Siswoyo Siswoyo Zulfikar Zulfikar