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Uji Daya Hambat Ekstrak Kasar Bunga Telang (Clitoria Ternatea L.) terhadap Bakteri Aeromonas Hydrophila secara in Vitro: In Vitro Inhibitory Test of Telang Flower (Clitoria Ternatea L.) Crude Extract Against Aeromonas Hydrophila Bacteria Aprilyanti, Salsabila; Andayani, Sri; Suprastyani, Heny
JFMR (Journal of Fisheries and Marine Research) Vol. 8 No. 3 (2024): JFMR on November
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2024.008.03.11

Abstract

Penyakit pada ikan sering menjadi kendala dalam kegiatan budidaya. Salah satu jenis bakteri patogen yang sering menginfeksi ikan air tawar yaitu bakteri Aeromonas hydrophila yang merupakan bakteri patogen yang menyebabkan penyakit Motile Aeromonas Septicemia (MAS). Upaya pengobatan menggunakan antibiotik dalam jangka waktu panjang akan menimbulkan residu dan resistensi bagi bakteri. Pengobatan alternatif yang dapat dilakukan yaitu dengan memanfaatkan senyawa bioaktif pada bahan alami yang bersifat antibakteri. Senyawa tersebut dapat ditemukan pada beberapa jenis tumbuhan, salah satunya yaitu bunga telang (Clitoria ternatea). Penelitian ini memiliki tujuan untuk mengetahui pengaruh dari ekstrak kasar bunga telang (C. ternatea) terhadap daya hambat bakteri A. hydrophila secara in vitro serta untuk menentukan dosis yang efektif dari ekstrak bunga telang. Metode yang digunakan pada penelitian ini yaitu metode eksperimen dengan Rancangan Acak Lengkap (RAL) dengan 5 perlakuan, 3 ulangan dan 2 kontrol dengan uji MIC (Minimum Inhibitory Concentration) dan uji cakram. Dosis ekstrak kasar bunga telang (C. ternatea) yang digunakan yakni 125 ppm, 150 ppm, 175 ppm, 200 ppm, dan 225 ppm. Penelitian ini menggunakan kontrol berupa antibiotik tetracycline dengan dosis 30 ppm dan kontrol negatif hanya berupa akuades. Hasil yang diperoleh dalam penelitian ini ialah pemberian ekstrak kasar bunga telang (C. ternatea) berpengaruh terhadap daya hambat bakteri A. hydrophila secara in vitro. Hasil tertinggi pada penelitian ini diperoleh pada dosis 225 ppm dengan rerata zona hambat 10,80 mm dengan persamaan y = 4,0713 + 0,0309x.   Fish diseases are often an obstacle in aquaculture activities. One type of pathogenic bacteria that often infects freshwater fish is Aeromonas hydrophila, which is a pathogenic bacteria that causes Motile Aeromonas Septicemia (MAS). Treatment efforts using antibiotics in the long-term will cause residues and resistance for bacteria. Alternative treatment that can be done is by utilising bioactive compounds in natural materials that are antibacterial. These compounds can be found in several types of plants, one of which is telang flower (Clitoria ternatea). This study aims to investigate the effects of crude extract from telang flower (C. ternatea) on the inhibition of A. hydrophila bacteria in vitro, as well as to determine the effective dosage of telang flower extract. The method used in this study is the experimental method with a Completely Randomized Design (CRD) with 5 treatments, 3 replications, and 2 controls with the MIC (Minimum Inhibitory Concentration) test and disc test. The doses of crude extract from telang flower (C. ternatea) used were 125 ppm, 150 ppm, 175 ppm, 200 ppm, and 225 ppm. The study utilized tetracycline antibiotic as a control at a dose of 30 ppm, and a negative control consisting solely of distilled water (aquades). The results obtained from this study indicate that the administration of crude extract from telang flower (C. ternatea) significantly affects the inhibition of A. hydrophila bacteria in vitro. The highest result in this study was achieved at a dose of 225 ppm with an average inhibition zone of 10,80 mm, described by the equation y = 4,0713 + 0,0309x.
Antibacterial Activity of Ethyl Acetate Fraction of Butterfly Pea Flower (Clitoria ternatea) Against Aeromonas hydrophila in vitro Aprilyanti, Salsabila; Andayani, Sri; Rahardjo, Seto Sugianto Prabowo
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 6 No. 2 (2025): Vol. 6 No. 2 (2025): JSMARTech Volume 6, No 2, 2025
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2025.006.02.33

Abstract

Aeromonas hydrophila is a pathogenic bacterium responsible for significant losses in freshwater aquaculture. In response to the growing issue of antibiotic resistance, this study examined the antibacterial activity of the ethyl acetate fraction of butterfly pea flowers (Clitoria ternatea) against A. hydrophila in vitro. Extraction was performed using ethyl acetate, followed by Thin Layer Chromatography (TLC) and column chromatography, which yielded nine fractions. Among them, fraction 5 showed the highest antibacterial activity. Phytochemical screening revealed the presence of alkaloids, tannins, flavonoids, and triterpenoids. The Minimum Inhibitory Concentration (MIC) test revealed that 250 ppm was the lowest effective concentration, while the disk diffusion assay demonstrated that 350 ppm produced the largest inhibition zone (10.03 ± 1.27 mm), which was statistically comparable to that of chloramphenicol (F = 91.34; p < 0.01). Scanning Electron Microscopy (SEM) revealed structural damage to A. hydrophila cells, including membrane disruption and lysis. This study introduces a new approach utilizing the ethyl acetate fraction of C. ternatea, combined with fractionation techniques and SEM imaging, to evaluate antibacterial activity. It offers potential for the development of environmentally friendly natural antibacterial agents for the aquaculture industry Keywords: Clitoria ternatea, Aeromonas hydrophila, ethyl acetate fraction, antibacterial activity.