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ISOLASI DAN IDENTIFIKASI JENIS JAMUR PADA UBI KAYU (Manihot esculenta Crants.) DALAM PROSES PEMBUATAN UBI KAYU HITAM SECARA TRADISIONAL OLEH MASYARAKAT BANDA Smith, Alwi; Hursepuny, Agnes
Biopendix: Jurnal Biologi, Pendidikan dan Terapan Vol 1 No 2 (2015): Biopendix : Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol1issue2page171-175

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Background: One of the roles fungi also in the manufacture of cassava black is one of the food products in addition to the public Banda, cassava black produced by cassava (Manihot esculenta Crants.) The levels of HCN (Hydrogen Cyanide) high through a process of soaking and fermentation drying, assisted by a fungus that is capable of binding the HCN. Methods: The process of making cassava samples conducted in Banda black. Isolation and identification of fungi carried on laboratory Pattimura University Faculty of Teacher Training and Education Science Biology Study Program Ambon. Results: from isolation in cassava (Manihot esculenta Crantz) in the process of making cassava traditional black communities Banda fungal isolates obtained 5 with 3 different genera is Rhizopus, Aspergillus, and Penicillium. Based on observations of macroscopic and microscopic morphology isolat1 belonging to the genus Rhizopus, isolat2, isolat3, isolat4 belonging to the genus Aspergillus and isolat5 belonging to the genus Penicillium. Results of macroscopic observation while hanging in the manufacturing process cassava spread of colonies of black mold growing cassava which dominates in the genus Rhizopus and Aspergillus genus. Conclusion: Based on research results isolation and identification of fungi that grow on cassava (Mahinot esculenta Crantz) in the process of making cassava traditionally black community can Banda in 5 isolates identified are included in 3 different genera is Rhizopus, Aspergillius, and penicillium.
The Mitotic Index of Cajanus cajan from Kisar Island, in the Southwest of Maluku Sangur, Kristin; Smith, Alwi; Tomasoa, Meike
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 2 (2021): August 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i2.29496

Abstract

The mitotic index of the roots of pigeon pea can be the basis for determining the growth of pigeon pea. The purpose of this research was to determine the time of root cell division, to observe the mitotic phases, and to determine the mitotic index of pigeon pea root cells. The preparation of the pigeon pea was carried out for 4 days to grow the roots. The roots were cut off at 08.00, 08.15, and 08.30 WIT (Eastern Indonesian Time). The roots were cut 0.5-1cm. Carnoy’s solution was used as the fixative solution using the Squash technique. The prepared roots were then observed using an Olympus cx-22 microscope and an OptiLab camera with a magnification of 100x40. The data were descriptively analyzed to describe the images of mitotic phases and the mitotic index presentation in the root cells of pigeon pea. The results of this research showed that the cell division of the pigeon pea roots began at 08.00 WIT, which was marked by the presence of a lot of prophase. The next phases that appeared were prometaphase, metaphase, and anaphase which occurred from 08.15 to 08.30 with different numbers. The highest mitotic index occurred at 08.15, when most of the root cells underwent metaphase. This study succeeded in revealing that the optimum time for pigeon pea root cell division is 08.15 WIT. In the future, this research can help pigeon pea farmers in Southwest of Maluku to carry out vegetative reproduction which is closely related to this mitotic study.
PENGARUH VOLUME PENYIRAMAN PUPUK CAIR URINE SAPI TERHADAP PERTUMBUHAN DAN PRODUKSI TANAMAN CABAI MERAH BESAR (Capsicum annum L) Ona Sitra Adu; Alwi Smith
BIOPENDIX Vol 2 No 1 (2015): Biopendix : Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol2issue1page20-32

Abstract

Background: Organic fertilizers is able to maintain the balance of the land and improve land productivity as well as reducing the environmental impact of land. Pepper plants contain lots of vitamin A and vitamin C and contain essential oils capsaicin, which causes a spicy flavor and heat to provide warmth when used for spices (herbs). Methods: The materials used in this study is the land of black, red chilli seeds large, EM4, water immersion soy, turmeric and wastewater cow urine. The study was conducted over three months, from April 2015 - July 2015. This study used a completely randomized design (CRD) with a liquid treatment factors limbar cow urine at levels as follows: P0 = control, ML P1 = 25, P2 = 50 ML and P3 = 75 ML. Each treatment was repeated 3 times. Data were analyzed using analysis of variance and if there is an influence, it will be followed by Least Significant Difference Test (BNT) at significance level of 5% and 1%. Results: The results showed that administration of cow urine liquid waste significant effect on the growth of large red pepper plant, that plant height, fruit number and weight of the fruit harvest. Conclusion: The growth of large red pepper plant with the highest scores were obtained in treatment P3 with a concentration of 75 ml while the growth to the lowest value was obtained in treatment P1 with a concentration of 25 ml.
IDENTIFIKASI DAN PEMANFAATAN JENIS UBI-UBIAN DI DESA ILMARANG KECAMATAN DAWELOR DAWERA KABUPATEN MALUKU BARAT DAYA Wilhelmina Lekawael; Alwi Smith
BIOPENDIX Vol 2 No 2 (2016): Biopendix : Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol2issue2page127-132

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Background: Cassava (Dioscorea sp.) Is a seasonal crop with twisted stems and forming yam. Sweet potatoes contain a large amount of carbohydrates, can be the basic ingredients of making flour, ethanol or alcohol. Method: The population in this study were 25 community-owned yams. This research conducted for 1 month from January 6 to February 6, 2016. Variable in this research is identification of type of sweet potato in Ilmarang Village. Result: Identification of 25 community gardens found 4 types of potatoes consisting of: cassava (Manihot esculenta Crants), sweet potato (Ipomea batatas L.), yam taro (Colocasia esculeata L.) and yam gembili (Diascorea esculenta L). Conclusion: Utilization of cassava as staple food of rice substitute and processed into various kinds of products such as crackers and food
KANDUNGAN SENYAWA FLAVONOID PADA DAUN MELINJO (Gnetum gnemon L.) BERDASARKAN PERBEDAAN TEMPAT TUMBUH Mersy T Tanamal; Pamella Mercy Papilaya; Alwi Smith
BIOPENDIX Vol 3 No 2 (2017): Biopendix : Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol3issue2page142-147

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Background: The village of Latuhalat and Kayu Putih village is a place that is on the beach and in the mountains. Potential natural resources are quite a lot of plants melinjo (Gnetum gnemon L). This study aims to determine the total compound of flavonoid content of leaf melinjo (Gnetum gnemon L) based on place difference. Method: The method used in this study using the method of Chang et al. The solvent used for diluting the melinjo extract was methanol solvent, then measured using a UV-VIS spectrophotometer. Result: Result of analysis of flavonoid compound content, Latuhalat Village has average total flavonoid content of13.080% and average total flavonoid content in Kayu Putih Village equal to 17.028%. Kayu Putih Village has more flavonoid compound than Latuhalat Village. Conclusion: Total levels of melinjo leaf flavonoids (Gnetum gnemon L) in Kayu Putih Village were more than total flavonoids of leaf melinjo (Gnetum gnemon L) in Latuhalat Village.
PERBEDAAN KADAR VITAMIN C DAN LAMA FERMENTASI TERHADAP YOGHURT DENGAN PENAMBAHAN SARI BUAH SIRSAK (ANNONA MURICATA L) Palijama Putri Selibata; Alwi Smith; H Sinay
BIOPENDIX Vol 4 No 1 (2017): Biopendix : Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol4issue1page44-47

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Background: Yogurt has a higher nutritional value than fresh milk because of the increase in total solids, so that the content of other nutrients also increases. In addition, yogurt is suitable for people with lactose intolerance or who are intolerant of lactose. One part of the soursop plant that can be used as medicine is fruit. Soursop fruit contains vitamins, dietary fiber, and antioxidant compounds that can function as compounds that can delay, slow down and prevent lipid oxidation. Method: This study was conducted at the Basic Biology and Basic Chemistry Education Laboratory of the Faculty of Mathematics and Natural Sciences, Pattimura University. In this study the number of treatments was 4 with different concentrations, the control group, the addition of soursop juice 30 ml, 60 ml and 100 ml. Results: Research shows that the more the concentration of soursop juice is added, the higher the content of vitamin C produced in the cow's milk yogurt. Conclusion: Different amounts of yoghurt with the addition of soursop juice have different levels of vitamin C. The highest average vitamin C is found in yogurt with the addition of 100 ml of soursop juice.
PEMANFAATAN ETNOBOTANI JENIS-JENIS TANAMAN OBAT DI DUSUN WAINUSALAUT DESA SULI KECAMATAN SALAHUTU KABUPATEN MALUKU TENGAH DAN IMPLIKASINYA SEBAGAI BAHAN AJAR MATA KULIAH BOTANI TUMBUHAN TINGGI Ruslia Pulu; Alwi Smith
BIOPENDIX Vol 5 No 1 (2018): Biopendix : Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol5issue1page13-17

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Background: Medicinal plants are all plants that can be used as medicine, ranging from those seen by the eye to those that appear under a microscope. Medicinal plants are all types of medicinal plants that are known or believed to have medicinal properties, which are grouped into traditional medicinal plants. The purpose of this study was to determine the types of medicinal plants used by the people of Wainusalaut Village as traditional medicine. Method: This type of research is qualitative with survey and interview techniques. Results: Research shows that 24 types of medicinal plants have been known to have been cultivated by the people of Wainusalaut Village and which have the potential as a drug as many as 18 species of plants that enter into 20 families. The part that is often used by the villagers of Wainusalaut is stems, roots, leaves and fruit. Conclusion: There are 24 types of medicinal plants that have been cultivated by the people of Wainusalaut Village
PENGARUH MODEL LEARNING CYCLE TERHADAP HASIL BELAJAR PADA MATERI PENCEMARAN LINGKUNGAN SISWA KELAS VII DI SMP NEGERI 15 AMBON Astrid Rhita Jacob; Alwi Smith
BIOPENDIX Vol 5 No 2 (2019): Biopendix : Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol5issue2page89-95

Abstract

Background: Education that is used as a forum to develop human potential requires a clear and mature set of systems to be able to deliver people to their potential development. For this reason, development in the education sector must continue to be developed in the direction of improving the quality of education. Method: This study was conducted at SMP Negeri 15 Ambon with a total of 25 students in class VII8. The KKM of SMP 15 Ambon biology subjects is 70. This study uses descriptive analysis to determine the learning outcomes of cognitive, affective, and psychomotor students. Results: Research shows that mastery of biological concepts before the application of the learning cycle model is classified as very low. This can be seen from the average score of achievement in the initial test, which is 40 from KKM. After the application of the learning cycle learning method, the formative test results obtained by students became better with the average percentage of achievement in the formative test increasing by 89. Conclusion: Learning cycle learning method is able to improve student learning outcomes on the concept of environmental pollution
PENAMBAHAN EKSTRAK DAUN KEMANGI DAN LAMA PERENDAMAN TERHADAP MUTU DAN DAYA AWET IKAN NILA (Oreachromis niloticus) SEGAR Enjel Souhoka; Alwi Smith; Ine Airini
BIOPENDIX Vol 6 No 1 (2019): Biopendix : Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol6issue1page7-11

Abstract

Background: Nila is a tropical fish that likes shallow water. Nila has better nutritional content compared to other freshwater fish, but Nila is also a food that is quickly damaged and spoiled. Pharmacological testing shows that basil has antibacterial activity. Basil leaves contain saponins, flavonoids and tannins which have many benefits besides being a spice in cooking it is also beneficial for bodily health. Method: This study immersed Nila in basil leaf extract (30 ml, 60 ml, 90 ml) dissolved in water to a volume of 0.5 liters for 30 minutes. After that it is stored at room temperature for 0 hours, 4 hours and 6 hours. Results: The results of the study using the ANOVA test showed that with the addition of basil leaf extract T1 (30ml), T2 (60 ml) and T3 (90 ml) there was no change in texture, color, odor and eyes on the 4 hour immersion while in the 6 hour immersion there was a change in texture and color. Conclusion: Based on the results and discussion above, it can be concluded that the addition of basil leaf extract to the freshness of Nila can be seen in 4 hours of immersion because, the results obtained are good, there is no change in the texture, color, odor and eye categories based on 4 sensory test scales. This happens because there is no enzyme, microorganism and chemical activity so that the freshness of the fish is maintained.
PERBEDAAN HASIL BELAJAR IPA BIOLOGI MENGGUNAKAN MODEL PEMBELAJARAN PROBLEM BASED LEARNING (PBL) DAN GROUP INVESTIGATION (GI) Ester Stasya Lawalata; Alwi Smith; S Liline
BIOPENDIX Vol 6 No 1 (2019): Biopendix : Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol6issue1page12-20

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Background: One of the causes of students' lack of understanding of a concept is teacher-centered learning and a lack of motivation from students in following the learning process. Then we need an innovative learning model that can help students in the learning process. To achieve learning objectives, teachers are required to be able to choose suitable learning models to be applied at an educational level. Method: This research was conducted from January 14 to February 14, 2020 using descriptive methods, student learning outcomes are seen through the final value that is seen based on cognitive aspects (LKS), affective and psychomotor and pre-post test scores. Measurement data were analyzed using the t test formula. Results: Based on the t test, obtained the results of tcount 1.573 and ttable 2.010, tcount <ttable, the hypothesis (H1) was rejected and (H0) was accepted. Conclusion: There is no difference in the learning outcomes of students taught using the Problem Based Learning model and Group Investigation on the material of the human digestive system in class VIII SMP Negeri 7 Ambon, but there is an increase in learning outcomes after teaching and learning using the Problem Based Learning and Group Investigation models so the results of the t-test show that tcount <ttable (1.573 <2.010).
Co-Authors Adelheid Adonia Erubun ARINI, INE Astrid Rhita Jacob Ayu Christine Rahaweman Baysi, Asmawati Rahma Cinde, Yuliante Dina Ismail Dwilestari, Desi Armawati Eclesia Danisa Eifan Boyke Pattiasina Eifan Boyke Pattiasina Elly, Sovian Sesca Enjel Souhoka Ester Stasya Lawalata Ferymon Mahulette Ferymon Mahulette, Ferymon Fredy Leiwakabessy Fredy Leiwakabessy H Sinay Hadi Wael Haulussy, Momina Haurissa, Ludia Hursepuny, Agnes Ince Wattimury Ine Airini Ine Arini Johanis F Rehena Kapludin, Yusran Karepesina, Fitriah Husna Kilay, Netty Charla Kristin Sangur Kristin Sangur Kristin Sangur Kristin Sangur Kristin Sangur Lekahena, Viona Putri Liline, Sintje Louvenska Nona Latupeirissa M Nur Matdoan Manuhuttu, Debi Lisa maria kiliroong Maulany, Grisendy Meike Ocktavia Lawalata Meike Tomasoa Mersy T Tanamal Meyer, Julyan Molle, Dessy Fitri Muh Nur Matdoan Muhammad Tarmizi Kubangun Muskitta, Marike Nanda, Dea Ariska Ona Sitra Adu Palijama Putri Selibata Pamella Mercy Papilaya Pamella Mercy Papilaya Papilaya, Pamella Mercy Persulessy, Audriel Eluzay Raharusun, Ayu Ningsih Rehena, Johanis Fritzgal Renyaan, Belsefren Ritha Karuwal Ritha Lusian Karuwal Rufiati Simal Rufiati Simal Rufiaty Simal Ruslia Pulu S Liline Sahetapy, Solagratia Salmanu, Sriyanti Imelda Aksamina Sangur, Kristin Silahoy, Frisca Simal, Rufiati Siti Mia Soulissa Sya’diyah, Zumrotus Syahran Wael Tetiwar, Welmina Tomasoa, Meike Tuanakotta, Risca M Tuapattinaya, Prelly M J Tuaputty, Hasan Wa Ode Zulfiana Wilhelmina Lekawael Wuarbanaran, Ona Adelci Yasir Pikahulan