Article Per Year (5 Year)
p-Index From 2020 - 2025
0.408
P-Index
This Author published in this journals
All Journal Journal of Multidisciplinary Applied Natural Science
Maria Erna Kustyawati
Department of Agricultural Product Technology, University of Lampung, Bandar Lampung-35145 (Indonesia)
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Physics

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
In vivo Evaluation of Saccharomyces-Modified Tempeh as Potential Prebiotic and Probiotic Food using Mus musculus as an Animal Model Maria Erna Kustyawati; Tegar Suryawan; Samsul Rizal; Esa Ghanim Fadhallah; Khairun Nisa Berawi
Journal of Multidisciplinary Applied Natural Science Vol. 5 No. 1 (2025): Journal of Multidisciplinary Applied Natural Science
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.242

Abstract

Saccharomyces-modified tempeh (SM tempeh), which is produced by adding Saccharomyces cerevisiae during soybean fermentation, is considered to have the potential as a source of prebiotics. The research aims to determine the prebiotic activity score (PAS) of SM tempeh extract against the probiotics S. cerevisiae and Lactobacillus casei, as well as to evaluate the resistance of S. cerevisiae and Escherichia coli in the intestines of mice fed tempeh. The PAS evaluation was carried out using a factorial complete randomized block design with three replications and one-way ANOVA for data analysis followed by the least significant difference test (5%). Meanwhile, microbial survivability was carried out in vivo using male Mus musculus strain mice fed standard feed, and standard feed with tempeh extract supplementation. The results showed that the supplemetation of either SM or commercial tempeh extract to the growth media significantly affected on the microbial load of S. cerevisiae, L. casei and E. coli, but the concentrations of tempeh extract had no significant effect. Apart from that, the concentrations of tempeh extract had no effect on the PAS of S. cerevisiae and L. casei, meaning that it was able to promote the growth of probiotics in the amount added to the media in the range of 2–10%. In addition, the feeding type had a significant effect on the survival of S. cerevisiae and E. coli in the intestines. S. cerevisiae carried on SM tempeh was detected surviving in the mice intestine at a rate of 6.12 log CFU/g, indicating that the tempeh was a probiotic food. Most likely SM tempeh is a synbiotic food.
Extraction and Activity Test of Antibacterial Compounds from Limberry Leaves (Triphasia Trifolia) Against Vibrio Sp. Novita Herdiana; Yosua Musada Sagala; Samsul Rizal; Maria Erna Kustyawati
Journal of Multidisciplinary Applied Natural Science Vol. 5 No. 1 (2025): Journal of Multidisciplinary Applied Natural Science
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.244

Abstract

Limberry (Triphasia trifolia) is one of traditional medicinal plants whereas its leaves are known for various uses, one of which is as an antibacterial agent. The most common bacteria found in marine waters is Vibrio sp. This work seeks to distinguish antibacterial compounds inside the ethanol extract of limberry leaves and assess its repressive effect on Vibrio sp. The research was conducted with three repetitions and five different concentration levels: D1 (5%), D2 (10%), D3 (15%), D4 (20%), and D5 (25%). The observational data were analyzed using a completely randomized design (CRD) analysis of variance, followed by the least significant difference (LSD) test at a 5% significance level. Phytochemical screening revealed that the ethanol extract of limberry leaves contained antibacterial compounds namely flavonoids, tannins, steroids, alkaloids, and phenolics. UV-Vis spectrophotometry analysis indicated that the ethanol extract of limberry leaves had absorption at wavelengths of 202, 211, 228, 253, 270, and 323 nm. FTIR analysis showed that the ethanol extract of limberry leaves contained some functional groups, i.e., O–H, C–H alkanes, C–H alkenes C≡N, C=O, C=C, C–O esters, and C–O ethers. The inhibitory test also demonstrated that the ethanol extract of limberry leaves could inhibit the growth of Vibrio sp., with inhibition zone diameters formed at each concentration of 5%, 10%, 15%, 20%, and 25% being 7.280, 7.640, 7.713, 8.027, and 8.070 mm, respectively.
Co-Authors Esa Ghanim Fadhallah Khairun Nisa Berawi Novita Herdiana Samsul Rizal Tegar Suryawan Yosua Musada Sagala