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Inthasan, Jiraporn
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Economics, Econometrics & Finance Social Sciences

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EFFICIENCY OF MICROORGANISM IN YARDLONG BEAN (Vigna sesquipedalis (L.) Fruw.) PRODUCTION IN NORTHERN THAILAND Dechjirarattanasiri , Chatprawee; Inthasan, Jiraporn
Agric Vol. 36 No. 2 (2024)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2024.v36.i2.p283-292

Abstract

Biofertilizer is one of the substances that consist of living microorganismssuch as bacterial, actinomyces and fungi. These could be helped plants increasing essential elements and encourage growth. Rhizobium are a type of bacteria that conformation a symbiotic relationship with legume plant. This bacteria not only fix nitrogen but also are involved in increasing nutrient availability and regulating plant growth hormones. Mycorrhiza fungi are help plant by enhancing absorption and increasing uptake of nutrients. These beneficial of rhizobium and mycorrhiza bring this study to chose for checking the influence of yardlong bean production in Chiangmai, Thailand from June to November 2022. Randomized Completely Block Design (RCBD) with 4 treatments 4 replications as followed treatment 1) the control (without biofertilizer), treatment 2) rhizobium (R), treatment 3) mycorrhiza (M), and treatment 4) rhizobium + mycorrhiza (R + M) was laid out in this experiment. The data indicated that combining between rhizobium and mycorrhiza caused the highest average weight per pod (23.1 g) and pod length (53.4 cm), yardlong bean quality as well as vitamin C, total Chlorophyll and total dietary fiber at 20.2 mg 100g-1, 0.20 μg g-1 and 2.7 mg 100g-1 respectively. This biofertilizer management methods provided the higher nitrogen, phosphorus, potassium, calcium, magnesium iron and zinc contents of bean comparing with control treatment. Remarkably, mixed rhizobium and mycorrhiza elevated the highest yield of yardlong bean (369 kg ha-1)
EFFECT OF TRYPTOPHAN AND PH LEVEL ON INDOLE-3-ACETIC ACID (IAA) PRODUCTION BY Bacillus spp. Inthasan, Jiraporn; Dechjiraratthanasiri , Chatprawee; Khamhung, Athipat
Agric Vol. 38 No. 1 (2026): in progress
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2026.v38.i1.p15-26

Abstract

Tryptophan and pH are environmental factors that convincingly influence microbial growth, metabolism, and metabolite production. This study aimed to find out how tryptophan and pH affect the production of Indole-3-acetic acid (IAA) by Bacillus spp. in Nutrient Broth (NB) medium. A Completely Randomized Design (CRD) with six treatments and three replications was used. The confirmation bacterial strains were Brevibacillus agri, Brevibacillus borstelensis (2 isolates), and Bacillus subtilis. The NB medium was adjusted to four pH levels (5, 6, 7, and 8) and supplemented with different concentrations of tryptophan: 0, 102, 150, 300, 450, and 600 mg/ml. The results after incubated showed that Brevibacillus agri was detected the highest IAA content at pH level 7.0 with 600 mg/ml of tryptophan (204 µg/ml). Whereas Brevibacillus borstelensis and Bacillus subtilis caused the maximum of IAA contents at pH level 8.0 with 600 mg/ml of tryptophan at 79.31 µg/ml and 35.82 µg/ml respectively.
Co-Authors Dechjirarattanasiri , Chatprawee Dechjiraratthanasiri , Chatprawee Khamhung, Athipat