Science literacy very important to trained in learning because it is needed in the process of solving problems in everyday life. This study aims to produce collaborative learning-based E-worksheet on Environmental Change Material to Train Grade X Students' Science Literacy Skills that are feasible based on validity, practicality and effectiveness. E-worksheet based on collaborative learning developed with 4D models include define, design, develop, disseminate. The targets in this study were 20 students of class X SMAN 1 Taman. The data were analyzed using validation sheet to determine the validity of E-worksheet, implementation observation sheet and response questionnaire to determine practicality, pre-test and post-test sheet to determine the effectiveness. The results showed that the E-worksheet based on collaborative learning to train science literacy skills was valid with a score of 98.7% in terms of didactic, construction and technical aspects. E-worksheet based on collaborative learning was also declared practical based on the implementation of 97.14% and the response results of 98.75% with very practical interpretation. E-worksheet based on collaborative learning is declared effective based on N-gain, which is an increase of 0.79 with high category and sensitivity of ≥0.30. The effectiveness is reviewed from the achievement of science literacy indicators, namely identifying scientific phenomena by 100%, formulating problems 89%, designing investigations 98%, analyzing the results of the investigation 81%, evaluating results of investigation 87% and drawing conclusions with score of 83% with very high interpretation and on the indicator of analyzing the results of nvestigation with high category.