Article Per Year (5 Year)
p-Index From 2021 - 2026
0
P-Index
This Author published in this journals
All Journal BERITA BIOLOGI
Siregar, AH
Research Center for Biology-Indonesian Institute of Sciences
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
PENGARUH PEMBERIAN ELISITOR JAMUR Phytium aphanidermatum (Edson) Fitzp. TERHADAP KANDUNGAN AJMALISIN PADA KULTUR KALUS BERAKAR Catharanthus roseus (L.) G. Don Aprianita, Aprianita; RR, Esyanti; Siregar, AH
BERITA BIOLOGI Vol 6, No 4 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4046.319 KB) | DOI: 10.14203/beritabiologi.v6i4.3448

Abstract

An experiment to study the effect of ellicitor derived from Phytium aphanidermatum On ajmalicine content of Catharanthus roseus root- callus culture has been conducted. Root-callus was induced  from left segment, and grew optimally on Zenk medium with the addition of  10-7 M BAP and 10-5 M NAA. Root-callus was subcultured three times, and followed with elicitation with elicitor derived from autoclaved  P. aphanidermatum. The concentration tested were 0.50, 0.5, 1.0 and 5.0 mg DW/ml, and harcested at 0,18,36 and 72 hours. The ajmalicine was analyzed qualitatively and quantitatively by using High Performance Liquid Chromatography (HPLC). The result showed that ajmalicine content was influenced significantly by concentration of ellicitor and harvesting time. A significant increase of ajamalicine content (153.29 ± 1.61 mg/g DW) was achieved by additon of 1 mg Dw/ml ellicitor after 36 hours incubation. 
Co-Authors Aprianita, Aprianita RR, Esyanti