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All Journal Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology
Kurniadi, Nadine
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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Production of Antioxidant Peptides from Snakehead Fish Using Batch and Continuous Enzymatic Hydrolysis Kardinan, Selma Aprilla; Yasni, Sedarnawati; Budijanto, Slamet; Kurniadi, Nadine; Sitanggang, S.TP, MSc, Azis Boing
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 19, No 1 (2024): May 2024
Publisher : :Agency for Marine and Fisheries Research and Human Resources, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.864

Abstract

Bioactive peptides are promising functional ingredients. Due to its high protein content, snakehead fish (Channa striata) extract (SHFE) is one of the suitable parent proteins for bioactive peptides. This study aimed to investigate the production of SHFE-based antioxidative peptides in a conventional batch and continuous system facilitated by an enzymatic membrane reactor (EMR). The effects of different proteases (Alcalase, Neutrase), substrate concentrations, and enzyme-to-substrate ratios were investigated in the batch process. Continuous hydrolysis was then performed under the optimum conditions obtained from the batch process. The optimum conditions based on the antioxidant capacity measured by DPPH and FRAP assays were employing Alcalase with a substrate concentration [S] of 3% (w/v) and an enzyme-to-substrate ratio [E]/[S] of 10% (w/w). Continuous operation was shown to have been performed over a prolonged period, based on the calculated fouling rate. Furthermore, filtration of the resulting permeate with a smaller membrane pore size (2-kDa) increased the antioxidant capacity. This study is expected to increase the production of functional ingredients in snakehead fish.
Co-Authors Kardinan, Selma Aprilla SEDARNAWATI YASNI Sitanggang, S.TP, MSc, Azis Boing Slamet Budijanto