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All Journal Bioma : Jurnal Biologi Indonesia Jurnal Biogenerasi
Hasna Dyah Kusumardani
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Languange, Linguistic, Communication & Media Other

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REVIEW: PRODUKSI LIMONENE MELALUI REKAYASA JALUR PENTOSA FOSFAT MENGGUNAKAN CYANOBACTERIUM Synechocystis sp. PCC 6803 Azar, Alda Wydia Prihartini; Hasna Dyah Kusumardani; Haris Maulani
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5344

Abstract

Limonene, a terpenoid compound found in various plants such as oranges, lemons, and mint, has numerous applications in different industrial fields, including as a fragrance, flavoring agent, and biofuel. Conventional limonene production relies on agricultural outputs vulnerable to fluctuations caused by diseases or climate change. Therefore, metabolic engineering using microorganisms offers an interesting alternative for more efficient and sustainable limonene production. This review article aims to summarize the procedures for limonene production through the modification of the pentose phosphate (PP) and methylerythritol 4-phosphate (MEP) biosynthetic pathways in the cyanobacterium Synechocystis sp. PCC 6803 to produce limonene. Limonene synthase (lims) obtained from C. limon and M. spicata plants was cloned and transformed into cyanobacteria to enhance limonene production. Experimental results showed that genes associated with the limonene biosynthesis pathway, including ribose 5-phosphate isomerase (rpi), ribulose 5-phosphate 3-epimerase (rpe), and geranyl diphosphate synthase (gpps), were successfully expressed in Synechocystis. This study demonstrates that Synechocystis can be an efficient microbial system for limonene and other isoprene compound production, offering a more stable and environmentally friendly alternative than agricultural-based production.
REVIEW: PRODUKSI LIMONENE MELALUI REKAYASA JALUR PENTOSA FOSFAT MENGGUNAKAN CYANOBACTERIUM Synechocystis sp. PCC 6803 Azar, Alda Wydia Prihartini; Hasna Dyah Kusumardani; Haris Maulani
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5344

Abstract

Limonene, a terpenoid compound found in various plants such as oranges, lemons, and mint, has numerous applications in different industrial fields, including as a fragrance, flavoring agent, and biofuel. Conventional limonene production relies on agricultural outputs vulnerable to fluctuations caused by diseases or climate change. Therefore, metabolic engineering using microorganisms offers an interesting alternative for more efficient and sustainable limonene production. This review article aims to summarize the procedures for limonene production through the modification of the pentose phosphate (PP) and methylerythritol 4-phosphate (MEP) biosynthetic pathways in the cyanobacterium Synechocystis sp. PCC 6803 to produce limonene. Limonene synthase (lims) obtained from C. limon and M. spicata plants was cloned and transformed into cyanobacteria to enhance limonene production. Experimental results showed that genes associated with the limonene biosynthesis pathway, including ribose 5-phosphate isomerase (rpi), ribulose 5-phosphate 3-epimerase (rpe), and geranyl diphosphate synthase (gpps), were successfully expressed in Synechocystis. This study demonstrates that Synechocystis can be an efficient microbial system for limonene and other isoprene compound production, offering a more stable and environmentally friendly alternative than agricultural-based production.
Comparative analysis of pollen morphology at three flowering stages of Trimezia martinicensis (Jacq.) Herb. (Iridaceae), an invasive species in Indonesia Alda Wydia Prihartini Azar; Isnaeni Nur Ardianti; Hasna Dyah Kusumardani; Sri Lestari; Riska Desi Aryani
Bioma Vol. 21 No. 2 (2025): Bioma
Publisher : LPPM Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/bioma.v21i2.55503

Abstract

Pollen morphology provides critical insights into plant reproduction, taxonomy, and evolutionary relationships. This study investigates pollen development in Trimezia martinicensis (Iridaceae), an invasive alien species in Indonesia, across three flowering stages: closed bud, developed bud, and mature flower. Vegetative and floral traits were documented alongside stage-specific palynological analyses. Pollen grains were examined under light microscopy to assess changes in orientation, shape, aperture, and exine architecture. Results revealed distinct morphological transitions, including spheroidal grains with monocolpate apertures at early stages, progressing to more rounded forms with refined wall structures in mature flowers. These findings highlight the dynamic nature of pollen morphology during anthesis and underscore its relevance for reproductive biology, taxonomy, and management of IAS in Indonesia.
Co-Authors Alda Wydia Prihartini Azar Azar, Alda Wydia Prihartini Haris Maulani Isnaeni Nur Ardianti Riska Desi Aryani SRI LESTARI