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All Journal Jurnal Akuakultur Indonesia Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Chen, Cheng-Ann
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Agriculture, Biological Sciences & Forestry Environmental Science

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Effect of different extenders and cryoprotectants on sperm viability of mud crab, Scylla tranquebarica for short term storage Zakaria, Siti Nor Fatihah; Hazwani, Nur; Chen, Cheng-Ann
Jurnal Akuakultur Indonesia Vol. 23 No. 2 (2024): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.23.2.168-175

Abstract

The aim of the present study was to determine the extenders such as calcium-free saline, Ringer’s solution and phosphate buffer and to determine the effect of different concentrations of cryoprotectants with different duration of exposure for short term storage of mud crab, Scylla tranquebarica sperm cells. A total of 30 mud crabs were used in the present study. In the present study, three treatments with three replicates were tested to determine the suitable extender. Besides, three treatments (dimethyl sulfoxide, glycine and glycerol) were tested to determine the suitable cryoprotectants. Eight treatments (25, 20, 16, 4, 2, -4, -20 and -80 °C) were assessed to determine the optimal cooling rate. The result showed the phosphate buffer solution produced the highest sperm viability (82.36 ± 5.84 %) after 60 min compared the Calcium free saline and Ringer’s solution. The results for sperm viability at -4 °C, -20 C and -80 °C after 24 hours were 95.63 0.13 % (5 % glycine), 91.51 ± 1.91 % (5 % DMSO) and 93.97 ± 0.43 % (5 % glycine). By using the 5 % glycine as the best cryoprotectant, the results for the sperm viability in 6 h, 12 h, 24 h, day 7, day 14 and day 21 at 80 °C were 95.17 ± 0.92 %, 94.11 ± 0.64 %, 93.31 ± 0.51 %, 90.84 ± 2.05 % and 82.82 ± 0.41 %. In a nutshell, the phosphate buffer and 5 % glycine were the best extender and cryoprotectant in the present study.
A New Record of Flathead Lobster Thenus indicus Leach, 1816 in Kota Kinabalu, Sabah (Malaysia) Based on Morphology and Genetic Identification, with Global Distribution Checklist: Thenus indicus in Sabah, Malaysia Radzi, Ihsan Hani; Chen, Cheng-Ann; Hajisamae, Sukree; Daning Tuzan, Audrey; Teng, Sing Tung
Journal of Tropical Life Science Vol. 15 No. 1 (2025)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/

Abstract

Members of the genus Thenus Leach 1816, commonly known as flathead lobsters, are significant contributors to global slipper lobster fisheries.  This study presents the first record of Thenus indicus Leach, 1816 (Crustacea: Decapoda: Scyllaridae) in Kota Kinabalu, Sabah, Malaysia, extending its known distribution to East Malaysia. Prior reports of T. indicus had only been confirmed in Peninsular Malaysia. Species identification was confirmed by mitochondrial DNA cytochrome c oxidase subunit 1 (CO1) sequencing, and morphological examination revealing a lack of spotting on the pereiopods, characteristic setaetion pattern of the second pereiopods, and prominent dentate features of the third maxillipeds. The third pereiopod merus length (ML3) was more than 0.45 times the carapace length (CL), which fulfilled one of the morphometric ratios for identification of this species. Intraspecific p-distances (0.0 – 2.51%, N=10) compared with GenBank reference sequences further validated this finding. A checklist and global map are included to illustrate updated T. indicus distributions. This report enhances the biodiversity and fisheries records of flathead lobsters within Southeast Asia, ensuring better resource management and conservation efforts.
Co-Authors Daning Tuzan, Audrey Hajisamae, Sukree Hazwani, Nur Radzi, Ihsan Hani Teng, Sing Tung Zakaria, Siti Nor Fatihah