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Activity of Ethanoic Extract of Ginger (Zingiber officinale) Against Pathogenic E. coli and P. aeruginosa Faris, Huda Natiq; Muttair, Riam Yousfe; Abd, Rawa Abdulkareem
Academia Open Vol 10 No 1 (2025): June (In Progress)
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/acopen.10.2025.10496

Abstract

Ginger (Zingiber officinale) extracts were tested for their antibacterial effectiveness in Thi-Qar province against harmful microorganisms including Escherichia coli and Pseudomonas aeruginosa. To prepare the plant materials for extraction with 95% ethanol, they were collected, cleaned, dried, and ground. The bactericidal activity of ginger extracts was tested using the agar well diffusion technique at concentrations of 25%, 50%, 70%, and 100% w/v. Independent sample t-tests and one-way analysis of variance were part of the statistical analysis. Strong antibacterial activity was shown at all concentrations (p < 0.05) in the data, with the highest effectiveness shown against both bacterial strains at 100% concentration. When it came to P. aeruginosa, ginger was more effective than E. coli. These results support the use of ginger in alternative treatments for bacterial diseases by highlighting its potential as a natural antibacterial agent Highlights: Ginger extracts show antibacterial effectiveness against E. coli and P. aeruginosa. Highest activity observed at 100% concentration using agar well diffusion technique. Highlights ginger's potential as a natural antibacterial alternative for bacterial diseases. Keywords: Walnut husks, iron removal, biosorption, Langmuir isotherm, thermodynamic study
The Effect of Silver Nanoparticles Manufacturd by Lactobacillus Bacteria on Some Bacterial Isolates Causing Gingivitis: Pengaruh Nanopartikel Perak yang Diproduksi oleh Bakteri Lactobacillus terhadap Beberapa Isolat Bakteri Penyebab Radang Gusi Faris, Huda Natiq; Ghyadh, Bushra Ali; AL-Muswie, Rana Talib
Indonesian Journal on Health Science and Medicine Vol. 1 No. 2 (2024): October
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/ijhsm.v1i2.36

Abstract

A Silver nanoparticles is considered to be able to kill multi resistant isolates due to its little dynamic effect on Microorganisms. The Gram-positive bacterium Lactobacillus bulgaricus was used in this investigation to manufacture NPs and evaluate their efficacy against certain pathogenic strains (Staphylococcus aureus, Staphylococcus mutans). For the production of nanoparticles, pure cultures of Lactobacillus bulgaricus were obtained from patients with gingivitis and dental caries and cultivated in "nuterient agar" broth. After centrifuging the Lactobacillus bulgaricus culture, cell-free supernatant containing silver ions was used. aqueous samples, and use a well-agar spread test to assess their antibacterial properties against bacterial strains obtained from the mouths of patients with dental caries and periodontitis. The disc diffusion method is used to create an antibiotic profile that targets certain harmful microorganisms. The main signal of the synthesis and characterization of the silver nanoparticles was a change in hue to a light yellow.The combination is dark brown in hue, as determined by visual and ultraviolet absorption spectroscopy. The results showed that AgNPs generated by Lactobacillus bulgaricus have antibacterial efficacy against certain types of bacteria. Compared to the studied antibiotics, our results indicate that Lactobacillus bulgaricus has a tremendous potential for creating AgNPs that are very powerful against bacteria. Red blood cells are not cytotoxically affected by silver nanoparticles at concentrations of 1.5 mg/ml Highlights: Silver nanoparticles show antimicrobial potential against resistant bacteria. Lactobacillus bulgaricus synthesized AgNPs tested via disc diffusion method. AgNPs demonstrated strong antibacterial effects without cytotoxicity at 1.5 mg/ml. Keywords: A Silver nanoparticles, Lactobacillus bulgaricus, pathogenic strains