<![CDATA[One way to reduce the value of beef imports nationally is to conduct a program to increase the number of beef cattle breeders at the smallholder scale by increasing the efficiency of cattle reproduction through artificial insemination technology. The purpose of the study was to determine the difference in the quality of fresh semen and frozen semen of Limousin bulls. The research method used exploratory laboratory. The results of macroscopic observations showed that the value of fresh semen volume was 4.4-7.2 ml., the results of color observations on fresh and frozen semen were all milky white. The consistency of fresh semen is thick while frozen semen is liquid. Measurement of pH in fresh and frozen semen showed a result of 6.7, while the results of fresh semen motility measurements obtained a value of 77% and in frozen semen showed a result of 41.5%. Abnormality of fresh semen showed a value of 1.30% while in frozen semen the result was 0.00%. The results of the examination of spermatozoa concentration in fresh semen were 1,426(106)/ml, while in frozen semen it was 26.6(106)/ml. The results of microscopic evaluation showed that there were five variables that were significantly different (p<0.05), namely volume, viscosity, motility, abnormality, and concentration, and there were two variables that showed no significant difference (p>0.05), namely color and pH. It was concluded that there are significant differences in the five variables measured but these differences are still within reasonable threshold limits according to the quality standards published by the National Standardization Agency in 2021.]]>
