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All Journal Indonesian Journal of Jamu
Izzati, Mumpuni Nur
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Medicine & Pharmacology

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Authentication of Java Turmeric (Curcuma xanthorrhiza) from Turmeric (Curcuma longa) Using a Combination of UV-VIS-IR Spectrum and Chemometrics Izzati, Mumpuni Nur; Syafitri, Utami Dyah; Mohamad Rafi
Jurnal Jamu Indonesia Vol. 10 No. 1 (2025): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v10i1.313

Abstract

Java turmeric (Curcuma xanthorriza) and turmeric (Curcuma longa) show similar colors, so they have the potential to be adulterated with each other, especially if they are presented in powder. This research aims to develop an analytical method for authenticating both types of samples with adulterant concentrations of 0.01% w/w and 0.005% w/w for the infrared range and 0.5 μg/g and 1 μg/g for the UV-Vis range. The pure sample was extracted for 40 minutes with 1:10 ethanol using ultrasonication. The extract was then concentrated using a rotary evaporator and freeze dryer. Adulterant samples were prepared by mixing both types of extracts. The absorption of the solution was measured at a wavelength of 200–800 nm and a wave number of 4000–400 cm-1. Multivariate analysis using partial least squares-discriminant analysis (PLS-DA) and soft independent modeling of class analogies (SIMCA) was performed on the spectra. PLS-DA has not been able to authenticate adulterated samples. However, SIMCA analysis can detect differences between pure curcuma and adulterated samples in the infrared range until a concentration of 0.005% w/w, while it can only authenticate correctly in the UV-Vis range until a 1 μg/g concentration.
Co-Authors M. Rafi Utami Dyah Syafitri