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Fredine E. S. Rares
Universitas Sam Ratulangi Manado, Indonesia
Aerospace Engineering Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Education Electrical & Electronics Engineering Engineering Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics

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Pola Bakteri Aerob pada Sampel Sputum dan Uji Sensitivitas Antibiotik Pasien Rawat Inap di Rumah Sakit Islam Sitti Maryam Manado Aiko Budiman; Heriyannis Homenta; Fredine E. S. Rares
Jurnal Syntax Admiration Vol. 6 No. 1 (2025): Jurnal Syntax Admiration
Publisher : Syntax Corporation Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46799/jsa.v6i1.2076

Abstract

Sputum is material expelled through the mouth from the respiratory tract via the coughing mechanism. Coughing can easily transmit pathogenic microorganisms. Microorganisms produced by coughing can infect anyone. Antibiotics are drugs used to treat infectious diseases caused by bacteria. The misuse of antibiotics leads to an increase in antibiotic resistance. This study aims to identify aerobic bacteria in sputum samples and to determine the antibiotic sensitivity of bacteria in sputum samples from inpatients at Sitti Maryam Islamic Hospital, Manado. The type of research is descriptive with a cross-sectional design, which involves bacterial identification using Gram staining, biochemical tests, and antibiotic sensitivity tests. The results showed 12 samples, consisting of 8 Gram-positive bacteria and 3 Gram-negative bacteria, with 5 types of bacteria identified: Staphylococcus sp., Staphylococcus aureus, Bacillus sp., Streptococcus sp., and Sphingomonas paucimobilis. The sensitivity test results indicated that Gram-positive bacteria were sensitive to meropenem, amikacin, and ciprofloxacin (100%) but resistant to ampicillin. Gram-negative bacteria were sensitive to gentamycin, amikacin, ciprofloxacin, sulfamethoxazole/trimethoprim, aztreonam, and meropenem but resistant to aztreonam. The most commonly found bacteria were Staphylococcus sp., Sphingomonas paucimobilis, and Bacillus sp., which were sensitive to meropenem, amikacin, and ciprofloxacin, but resistant to ampicillin and aztreonam.
Pola Bakteri Aerob pada Sampel Sputum dan Uji Sensitivitas Antibiotik Pasien Rawat Inap di Rumah Sakit Dr. J.H Awaloei Ribka Milkallah Sepang; Heriyannis Homenta; Fredine E. S. Rares
Jurnal Syntax Admiration Vol. 6 No. 1 (2025): Jurnal Syntax Admiration
Publisher : Syntax Corporation Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46799/jsa.v6i1.2077

Abstract

Cough with phlegm is the most common medical complaint that leads to referral to a pulmonary specialist. Based on the World Health Organization (WHO) report that every year 4 out of 13 million deaths in developing countries are caused by respiratory tract infections, and at Dr. JH Awaloei Hospital, respiratory tract infections are ranked 4th out of 10 most common diseases. ARI can be treated with antibiotics if the microorganisms that cause it are bacteria, but the use of antibiotics must be in accordance with antibiogram data, rational and appropriate doses to prevent an increase in antibiotic resistance, because antibiotic resistance has become a chronic and global public health problem. Purpose: This study aims to identify aerobic bacterial patterns and antibiotic sensitivity tests in sputum samples of inpatients at Dr. J.H Awaloei Hospital. Methods: This study used a descriptive method with a cross sectional approach, namely by conducting bacterial pattern research on 15 samples, then cultured using nutrient agar and mac conkey at the Microbiology Laboratory of the Faculty of Medicine, Sam Ratulangi University. Results: Identification of bacteria by Gram staining. Antibiotic sensitivity test. The results of this study obtained Staphylococcus sp. (86.67%); Staphylococcus aureus (6.67%); Streptococcus sp. (6.67%). All bacteria were sensitive to the antibiotic Amikacin and resistant to Ampicillin and Clindamicin. The conclusion of this study is that the most bacteria found are Staphylococcus sp. which is most sensitive to Amikacin, Meropenem and most resistant to Ampicillin, Aztreonam, Clindamicin, Erythromycin.
Co-Authors Aiko Budiman Heriyannis Homenta Ribka Milkallah Sepang