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Efektivitas Antioksidan Likopen Pada Buah Tomat (Lycopercisum esculentum) Terhadap Normalitas dan Abnormalitas Spermatozoa Sapi Bali dengan Metode Swim up Syuhriatin
Bionature Vol. 22 No. 1 (2021): April 2021
Publisher : Jurusan Biologi Universitas Negeri Makassar

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Abstract

Penelitian ini merupakan jenis penelitian eksperimen yangdilakukan di laboratorium dengan tujuan untuk mengetahui suatu gejalaatau pengaruh yang timbul sebagai akibat dari adanya perlakuan tertentu.Penelitian dilaksanakan di Laboratorium Imunobiologi Fakultas MIPAUniversitas Mataram. Materi yang digunakan dalam penelitian yatu 1 ekorsapi bali jantan yang berumur sekitar 3,5 tahun dengan berat badan sapi460 kg, yang ditampung semennya dengan menggunakan vagina buatan.Rancangan percobaan yang digunakan dalam penelitian ini adalahRancangan Acak Lengkap yang disusun dengan pola faktorial yang terdiriatas 3 (tiga) faktor. Faktor pertama adalah perbedaan lapisan (L) yangterdiri atas 2 (dua) taraf yaitu Lapisan Atas (LA) dan Lapisan Bawah (LB).Faktor kedua adalah konsentrasi likopen buah tomat (P) terdiri atas 5 (lima)taraf, yaitu: 0% (P0), 5% (P1), 10% (P2), 15% (P3) dan 20% (P4). Faktorketiga adalah lama penyimpanan (H) yang terdiri atas 6 (enam faktor), yaitu1 hari (H1), 2 hari (H2), 3 hari (H3), 4 hari (H4), 5 hari (H5) dan 6 hari (H6).Ketiga faktor tersebut bila dikombinasikan menghasilkan 60 kombinasiperlakuan, setiap kombinasi perlakuan diulang 6 (enam) kali sehinggaterdapat 360 satuan (unit) percobaan. Hasil analisis varians menunjukkan,bahwa penambahan antioksidan likopen buah tomat dan pemisahan denganmetode swim up tidak berpengaruh nyata (P>0,05) terhadap normalitas danabnormalitas spermatozoa. Penambahan antioksidan likopen buah tomatdan pemisahan dengan metode swim up tidak memberikan pengaruhterhadap peningkatan abnormalitas spermatozoa, namun tidak memberikanefek negatif pada spermatozoa sapi bali
Reduction of Nitrite Levels in Edible Bird Nest on Lombok Island with Alkali Solution and Oxygen Water Juniawan, Alvin; Andini, Arista Suci; Syuhriatin
Jurnal Penelitian Pendidikan IPA Vol 11 No 2 (2025): February
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i2.9682

Abstract

In this study, sampling was carried out at 3 locations of swallow nest farmers, namely in East Lombok, Central Lombok and West Lombok. The type of swallow nest used in this study was the Linchi swallow nest. This study was a quantitative study, namely to find the best variation in soaking time with alkaline water and Oxygen Water to reduce nitrite levels. Sample measurement using a Uv-Vis spectrophotometer instrument and characterization using FTIR. The results of the study showed the highest reduction in nitrite levels using an alkaline solvent with a pH of 7.5, Central Lombok, East Lombok and West Lombok Edible Bird’s Nest Sample, respectively, as follows: 87.88%; 96.26%; and 94.59% with an optimum contact time of 30 minutes. While the highest reduction in nitrite levels for Central Lombok, East Lombok and West Lombok, Edible Bird’s Nest samples were as follows: 92.51%; 98.5%; and 97.85% with an optimum contact time of 15 minutes. This is also supported by FTIR spectra data from optimum conditions for reducing nitrite levels where the characteristics of the FTIR spectra in the 1400 cm-1 wavenumber region have a %T value after treatment> to %T before treatment. The results of the macronutrient test showed that the bird's nest contains carbohydrates, proteins and lipids. While the results of the phytochemical test showed that the bird's nest sample contained phenolic compounds. In this study, it has been successfully reduced nitrite levels in bird's nests using alkaline solvents pH 7.5 and Oxygen Water which is the latest method that is cheaper and more efficient.
Co-Authors Alvin Juniawan Andini, Arista Suci