Agus Budi Setiawan
Laboratory of Plant Breeding, Department of Agronomy, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta

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Assessment of F₅ Mungbean Genotypes from Intergeneric Hybridization with Common Bean for Agronomic Performance, Heritability, Variance Components, and Genetic Uniformity Binti Nur Aisah; Aprilia Tiara Ikhwani; Agus Budi Setiawan; Aziz Purwantoro; Dyah Weny Respatie; Erlina Ambarwati; Listy Anggraeni; Chee How Teo; Chandra Setyawan; Aniswatul Khamidah
Caraka Tani: Journal of Sustainable Agriculture Vol 40, No 3 (2025): July
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v40i3.94563

Abstract

Genetic improvement in mungbean (Vigna radiata L. [Wilczek]) is constrained by its narrow genetic base. Introducing genetic material from common bean (Phaseolus vulgaris L.) through intergeneric hybridization offers a promising strategy for enhancing yield potential and advancing sustainable agriculture. However, limited studies have evaluated the agronomic performance and genetic parameters of progenies derived from such crosses. This study aimed to assess agronomic performance, estimate genetic parameters, and evaluate genetic uniformity in mungbean progenies, to identify superior lines for breeding programs. The field experiment was conducted using a randomized complete block design with four replicates. Five selected F₅ genotypes (C419, B423, B1922, B119, and B1124), along with the mungbean progenitor PKHPL-1, were evaluated. Traits assessed included vegetative, generative, and yield-related characteristics. Significant variation was observed among genotypes for yield-related traits, while plant height, number of flowers, and phenological traits showed relative uniformity. Genotype C419 exhibited the highest agronomic performance, with superior values in 100-seed weight, number of seeds per pod, seed weight per plant, and number of pods per plant. High heritability was recorded for pod length and 100-seed weight (90.63% and 90.32%, respectively), indicating strong genetic control and potential for effective selection. Principal component analysis confirmed the major contribution of these traits to yield variation. Furthermore, molecular analysis using IRAP markers revealed high genetic uniformity in genotype C419. These findings demonstrate the potential of intergeneric hybridization for enhancing yield-related traits in mungbean and identify genotype C419 as a promising line for future breeding efforts.
Genetic Variability in F2 Melon (Cucumis melo L.) Population from Double Cross of Sex-Distinct Parent Lines Agus Budi Setiawan; Irna Auliauzzakia; Aziz Purwantoro
Caraka Tani: Journal of Sustainable Agriculture Vol 40, No 2 (2025): April
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v40i2.93441

Abstract

Melon (Cucumis melo L.) is an economically significant crop in Indonesia. Due to the increasing demand for premium melon fruit with high sugar content, firm flesh, extended shelf life, bright peel and flesh color, and round fruit shapes, researchers developed melon lines that align with consumer preferences. This study aimed to determine the segregation pattern and genetic basis of sex expression in F1 and F2 populations, estimate genetic parameters for pericarp thickness and total soluble solids, and identify superior F2 genotypes using a predicted selection response based on a weighted selection index. A total of 137 F1 individuals derived from crossing ‘Inthanon RZ’ with ‘Glamour Sakata’ and 237 F2 individuals derived from the self-pollinated IG10 line were grown in a greenhouse using a hydroponic drip fertigation system. The F1 population exhibited genetic variation in sex expression based on the allele-specific marker of CmACS7, with a 1:1 phenotypic ratio, consisting of 68 monoecious and 69 andromonoecious individuals. All individuals in the F2 population showed homozygote andromonoecious expression, indicating that the IG10 progenitor line was homozygous (aaGG). Pericarp thickness and total soluble solids exhibited high phenotypic and genotypic coefficients of variation and moderate-to-high broad-sense heritability. Among the genotypes, four displayed high-weighted selection indices based on the two target traits, with IG10-124 achieving the highest selection index. The selection response based on the weighted selection index suggests that pericarp thickness and total soluble solids will show genetic improvement in the next generation.