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PENGARUH KONSENTRASI NATRIUM ALGINAT SEBAGAI PENJERAT SEL Lactobacillus acidophilus FNCC 0051 DAN LAMA PENYIMPANAN TERHADAP JUMLAH SEL YANG TERLEPAS DAN KARAKTER CARRIER Ratnasari, Nike; Kusumawati, Netty; Kuswardhani, Indah
Jurnal Teknologi Pangan dan Gizi Vol 13, No 2 (2014)
Publisher : Widya Mandala Surabaya Catholic University

The aim of this study is to know the effect of sodium alginate concentration in Lactobacillus acidophilus FNCC 0051 entrapment, refrigeration storage (Â±5oC) period and interaction of both factors on the number of released cells and carrierâ€™s character (UHT milk). The experimental design used was factorial RBD (Randomized Block Design) design with two factors, sodium alginate concentration (1%, 1,5%, 2%) and storage period (0,10, 20 days). The parameters observed are the number of released cells (Total Plate Count / TPC), pH and total lactic acid of the carrier (UHT milk). The obtained data are statistically analyzed by ANOVA (Analysis of Variance) at Î±=5% and continued with Duncanâ€™s Real Difference Distance test (Duncan's Multiple Range Test) to determine which level of the treatment that gives a significant differences. Sodium alginate concentration and storage period had significant effect on the number of released cells and carrierâ€™s character (pH and total lactic acid of UHT milk) and there is interaction between both factors. The lower level of sodium alginate concentration increased the number of released cells, decreased pH value and increased total lactic acid. During 20 days storage, the amount of released cells and acidity of carrier increased than 0 and 10 days. Interaction between both factors which give the less number of released cells (3,7948 log cfu/ml) and carrierâ€™s character changes (pH = 6,4767 and total lactic acid= 0,0741 %) is combination of 2% sodium alginate concentration in 0 days storage.

PENGARUH LAMA PENYIMPANAN SELAMA DISTRIBUSI DAN PEMASARAN TERHADAP VIABILITAS BAKTERI ASAM LAKTAT DAN TINGKAT KEASAMAN PADA YOGURT MURBEI HITAM (Morus nigra L.) Oktavia, Hana Meliana; Kusumawati, Netty; Kuswardhani, Indah
Jurnal Teknologi Pangan dan Gizi Vol 14, No 1 (2015)
Publisher : Widya Mandala Surabaya Catholic University

Yoghurt is the product from coagulation of milk that is produced from fermentation process of lactic acid bacteria (LAB), Lactobacillus bulgaricus (LB) and Streptococcus thermophilus (ST). Usually during storage and distribution, the viability of LB and ST decrease. Marketing and distribution method which is done by small scale industry is using combination storage on coolbox with 15oC-20oC during 10 hours and followed by storage on refrigerator with 5oC during 14 hours. The purpose of the research observe the effect of storage time on such condition. The research purposed to observe the effect of storage time during distribution and marketing on the viability of Lactic Acid Bacteria (ST and LB) and acidity levels in black mulberry yogurt. The research based on RBD (Randomized Block Design) single factor: the long of storage during distribution and marketing (1; 5; 9; 13; 17 and 21 days) with 4 replications. The parameters observed were pH, total acid and viability LAB (ST and LB). The data obtained were analyzed statistically with Analysis of Variance (ANOVA) and Duncan Multiple Range Test (DMRT) (Î± = 0.05). The long of storage during distribution and marketing gave significant affect on the viability of lactic acid bacteria and the acidity of the black mulberry yogurt. The longer of storage time, the lower LAB viability and pH, but higher total acid. The value of black mulberry yoghurt ALT LAB after 21 days of storage 9.1484 log cfu / ml with a pH value of yogurt 3.814, acidity Soxhlet Henkel 95.03 oSH and total of lactic 2.14%. Black mulberry yogurt during 13 days of storage and distribution is qualified to SNI with 9.5148 ALT log cfu/mL and a total of lactid acid 1.97%.

PENGARUH KONSENTRASI TEPUNG PEPAYA DAN LAMA PENYIMPANAN TERHADAP SIFAT FISIK BEADS DAN VIABILITAS Lactobacillus acidophilus FNCC 0051 TERIMOBIL Purba, Grace Sri; Widyastuti, Theresia Endang Widoeri; Kuswardhani, Indah
Jurnal Teknologi Pangan dan Gizi Vol 15, No 2 (2016)
Publisher : Widya Mandala Surabaya Catholic University

The purpose of this study was to determine the effect of papaya powder concentration and storage time to the physical properties of beads and viability of immobilized Lactobacillus acidophilus FNCC 0051. The Randomized Block Design (RBD) factorial design to be used. There were two (2) factors: concentration of papaya powder consisting of two (2) levels, which were 3% (w/v) and 6% (w/v) and storage time consisting of five (5) levels, which were 0, 7, 14, 21, and 28 days. Each treatment combinations repeated three (3) times. Testing is conducted on the beadsâ€™ diameter, beadsâ€™ texture (hardness, cohesiveness, springiness) and also immobilized cellâ€™s viability. Data analyzed by ANOVA test (Analysis of Varians) at Î± = 5% and continued with DMRT (Duncan's Multiple Range Test) at Î± = 5 if the level of treatment had a significant effect. Beads diameter ranged 5.07 â€“ 5.68 mm and the higher papaya powder concentration or the longer storage time would make the beadsâ€™s diameter bigger. Hardness ranged 29.2376 â€“ 48.5756 g, cohesiveness ranged 0.3800 â€“ 0.5211, and springiness ranged 0.8398 â€“ 0.9889. The higher level concentration of papaya powder decrease beadsâ€™ hardness and cohesiveness but thereâ€™s no effect on springiness. The viability of immobilized Lactobacillus acidophillus FNCC 0051 ranged 8.6021 â€“ 11.5474 log cfu/gram. Storage time significantly influenced the viability of immobilized cells, which the highest viable cells (11.1795 log cfu/g) obtained from 21 days of storage time.

PENGARUH PROPORSI SUKROSA-ISOMALT TERHADAP SIFAT FISIKOKIMIA DAN ORGANOLEPTIK PERMEN JELLY ANGGUR BALI (Alphonso lavalle) Susilo, Ivone; Suseno, Thomas Indarto Putut; Kuswardhani, Indah
Jurnal Teknologi Pangan dan Gizi Vol 12, No 1 (2013)
Publisher : Widya Mandala Surabaya Catholic University

Jelly candies has a clear transparent appearance and resilience texture with variety colors and sweet taste. Natural colorant can be used in jelly candies are anthocyanins contained in Bali grapes. Isomalt can be used as alternative sweetener in jelly candies as it can form the body and has a sweet taste without after-taste. Isomalt is more stable at higher temperatures and more resistant to hydrolysis by enzymes and acids than sucrose, but with a lower solubility. The different properties of sucrose and isomalt can cause changes in the physicochemical and organoleptic characteristics of jelly candies that need to be investigated the right proportion of sucrose-isomalt. The research design used is Randomized Block Design with a single factor. Isomalt concentrations used were 0%, 5%, 10%, 15%, 20%, 25% with four repetitions for each treatment. The parameters tested include the physicochemical properties of the water content, pH, texture (firmness and tensile strength) and organoleptic properties (color, flavor, and texture). Based on statistic analysis, different proportion of sucrose and isomalt gave significant effect (Î±=5%) on water content, firmness, elasticity, organoleptic test taste and texture but color was not significantly influenced. The higher proportion of isomalt increased water content and firmness of jelly candies, but decreased tensile strength. Treatment isomalt 20% gives the best result based on additive weighting, have 19,18% water content, 14,62 N/10 mm firmness, 119,14 mm tensile strength, the preferance score of color was 3,59, taste was 3,71, texture was 3,60.

KAJIAN JUMLAH SEL TERLEPAS DAN KARAKTER CARRIER PADA BERBAGAI KONSENTRASI TEPUNG PEPAYA DAN LAMA PENYIMPANAN SEL TERIMOBIL DALAM BEADS Christiane, Claudia; Widyastuti, Theresia Endang Widoeri; Kuswardhani, Indah
Jurnal Teknologi Pangan dan Gizi Vol 15, No 2 (2016)
Publisher : Widya Mandala Surabaya Catholic University

Papaya flour added as prebiotic can influencing the cell growth (Lactobacillus acidophilus FNCC 0051) and beads texture, also total released cells from the beads thus affecting carrier characteristic. The purpose of this research is to study the interaction of papaya flour concentration and storage time, also effect of each factor to total released cells and carrier characteristic. The research design used is Factorial Randomized Block Design (RBD) with two factors namely concentration of papaya flour consisting of two levels, which were 3% (w/v) and 6% (w/v) and the storage time consisting of five (5) levels, which were 0, 7, 14, 21, and 28 days. Each treatment combinations are repeated three times. The analyzed variables were total released cells, pH, total acidity from milk as carrier and also the total of immobilised cells. Data analyzed statistically using ANOVA test (Analysis of Varians) at Î± = 5% and continued with DMRT (Duncan's Multiple Range Test) at Î± = 5% to determine which level of treatment that gives significant differences. Papaya flour concentration and storage time give significant interaction effect to the total released cells and total acidity, also significant effect of each factor to carrierâ€™s pH. The higher concentration of papaya flour and the longer storage time increased the total released cells and total acidity. The significant increase of total acidity found in combination of various concentration of papaya flour with 14 to 28 days of storage. The higher concentration of papaya flour or the longer storage time decreased the pH value.

PENGARUH JENIS GULA DAN KONSENTRASI EKSTRAK THE HIJAU TERHADAP KARAKTERISTIK FISIKOKIMIA, VIABILITAS BAKTERI ASAM LAKTAT, DAN ORGANOLEPTIK YOGURT NON FAT Taniaji, Suzanna; Kusumawati, Netty; Kuswardhani, Indah
Jurnal Teknologi Pangan dan Gizi Vol 15, No 1 (2016)
Publisher : Widya Mandala Surabaya Catholic University

Yogurt is a fermented milk product which utilizes two kinds of lactic acid bacteria, Streptococcus thermophillus (ST) dan Lactobacillus bulgaricus (LB). Utilization of another kind of sugar such as isomalt, which has lower amount of calories compared to sucrose, may be used as an alternative sweetener in yogurt product. Addition of green tea extract is expected to act as natural colorant and aroma and flavor enhancer. The research used experimental design of Randomized Block Design (RBD) nested with two factors: the type of sugar and green tea extract concentrastion with 4 replications. The parameters observed are pH acidity, syneresis, total of lactic acid bacteria, and organoleptic properties (preferences of taste, aroma, and color). The data obtained were statistically analyzed by ANOVA test (Analysis of Variance) at Î±=5% and continued with Duncanâ€™s Multiple Range Test to determine the level of treatment which has significant difference. The selection of the best treatment is done by Effectiveness Index test. The use of different types of sugar have no significant effect on pH, acidity, syneresis, and total lactic acid bacteria (BAL), while the difference in the concentration of green tea extract was significantly different to acidity, syneresis, and total lactic acid bacteria. Differences in the concentration of green tea extract with isomalt resulted in increasing acidity, syneresis, and total lactic acid bacteria. Utilization of sucrose resulted significant difference in 15% green tea extract concentraion. The use of sucrose has higher acceptability compared to isomalt. Taste properties between sugar and isomalt yogurt is significantly different, while aroma and color is insignificant, which isomalt is in 'rather liked' category. 20% concentration of green tea extract is concluded to be the best treatment, with properties of sucrose-utilized yogurt are 4.532 pH, 0.70% acidity, 5.09% syneresis, 9.4605 log cfu/mL Total Plate Count (TPC), while properties of isomalt yogurt are 4.508 pH, 0.81% acidity, 8.44% syneresis, 9.8743 log cfu / mL TPC.

PENGARUH KONSENTRASI TEPUNG PEPAYA DAN LAMA PENYIMPANAN TERHADAP KETAHANAN L. acidophilus FNCC 0051 TERIMOBIL PADA KONDISI ASAM LAMBUNG DAN GARAM EMPEDU Sucipto, Mariska; Widyastuti, Theresia Endang Widoeri; Kuswardhani, Indah
Jurnal Teknologi Pangan dan Gizi Vol 15, No 2 (2016)
Publisher : Widya Mandala Surabaya Catholic University

Papaya powder added as a prebiotic, but its concentration and storage time in UHT milk as a carrier can impact on viability of immobilized cell in gastric acid and bile salt condition. The purpose of this study was to determine the effect of the interaction of papaya powder concentration and storage time and also the influence of each these factors on the survival of immobilized L. acidophilus FNCC 0051 in gastric acid and bile salt. The research design used a Randomized Block Design (RBD) of factorial design. Papaya powder concentrations as the first factor consisting of two levels (3% b/v and 6% b/v) and the storage times as the second factor consisting of five levels (0, 7, 14, 21, and 28 days). Each treatment combinations repeated three times. Data were analyzed by ANOVA (Analysis of Variance) at Î± = 5%. Based on the results, use of 6% concentration papaya powder significantly increasing the survival of immobilized L. acidophilus FNCC 0051 on condition of gastric acid. Meanwhile, interaction of papaya powder concentration and storage time significantly affect the resistance of immobilized L. acidophilus FNCC 0051 on bile salt condition. Storage along 14 days decreasing the cells number, but the survival cell on bile salt condition increased by the use of 6% concentration of papaya powder during 21 days of storage. The highest survival with decreased cell 0,1459 log cfu/g obtained from combination of 6% concentration of papaya powder at 21 days of storage time.

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