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Misnati Nur Indahsari, Wiwin
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EKSPLORASI DAN IDENTIFIKASI JAMUR TRICHODERMA SP DENGAN METODE PENGENCERAN SEBAGAI AGEN HAYATI DI LABORATORIUM AGENS HAYATI UPT PROTEKSI TANAMAN PANGAN DAN HORTIKULTURA JAWA TIMUR Fatimatul Ula, Siti; Misnati Nur Indahsari, Wiwin
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5627

Abstract

This study aims to determine the results of Trichoderma sp fungus exploration in healthy bamboo and chili horticulture land and dense chili and to determine the macroscopic and microscopic characteristics of Trichoderma sp fungus as a biological agent. This type of research is descriptive exploratory with tools and materials, namely Aquades, 70% alcohol, PDA media, potatoes, technical agar, dextrose, chloramphenichol, tomato soil samples, healthy chili soil, dense chili soil, bamboo soil, tissue, methylated spirits, LPG, plastic wrap, cotton, label paper. The tools used are hoes, scoops, rulers, PH meters, rapitest, Laminar air flow, Ose needles, Petri dishes, test tubes, Erlenmeyer flasks, L sticks, Vortex, Plastic, Plastic boxes, Bunsen burners, stoves, sterilization pans, autoclaves, tube racks, tweezers, microscopes, glass objects, cover glasses, scales, trays, 1cc syringes, black cloth. Based on the results of exploration and identification activities, Trichoderma sp fungi were obtained with the dilution method precisely at a dilution level of 10-7. In addition, Trichoderma sp fungi were found in tomato soil. The diversity of fungi in tomato plants depends on environmental conditions. The value of tomato rhizosphere diversity in non-endemic is H'> 3.
EKSPLORASI DAN IDENTIFIKASI JAMUR TRICHODERMA SP DENGAN METODE PENGENCERAN SEBAGAI AGEN HAYATI DI LABORATORIUM AGENS HAYATI UPT PROTEKSI TANAMAN PANGAN DAN HORTIKULTURA JAWA TIMUR Fatimatul Ula, Siti; Misnati Nur Indahsari, Wiwin
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5627

Abstract

This study aims to determine the results of Trichoderma sp fungus exploration in healthy bamboo and chili horticulture land and dense chili and to determine the macroscopic and microscopic characteristics of Trichoderma sp fungus as a biological agent. This type of research is descriptive exploratory with tools and materials, namely Aquades, 70% alcohol, PDA media, potatoes, technical agar, dextrose, chloramphenichol, tomato soil samples, healthy chili soil, dense chili soil, bamboo soil, tissue, methylated spirits, LPG, plastic wrap, cotton, label paper. The tools used are hoes, scoops, rulers, PH meters, rapitest, Laminar air flow, Ose needles, Petri dishes, test tubes, Erlenmeyer flasks, L sticks, Vortex, Plastic, Plastic boxes, Bunsen burners, stoves, sterilization pans, autoclaves, tube racks, tweezers, microscopes, glass objects, cover glasses, scales, trays, 1cc syringes, black cloth. Based on the results of exploration and identification activities, Trichoderma sp fungi were obtained with the dilution method precisely at a dilution level of 10-7. In addition, Trichoderma sp fungi were found in tomato soil. The diversity of fungi in tomato plants depends on environmental conditions. The value of tomato rhizosphere diversity in non-endemic is H'> 3.
EKSPLORASI SERTA IDENTIFIKASI BAKTERI DAN CENDAWAN TANAH KABUPATEN SAMPANG SEBAGAI AGENS HAYATI DI LABORATORIUM AGENS HAYATI UPT PTPH JAWA TIMUR Dyah Putri Yudha Yanti; Misnati Nur Indahsari, Wiwin
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v11i1.8149

Abstract

Exploration of soil microorganisms as biological control agents is one of the environmentally friendly approaches to controlling plant pests and diseases. This study aimed to explore and identify soil bacteria and fungi with potential as biological control agents at the Biological Control Laboratory of the Technical Implementation Unit for Food Crops and Horticultural Plant Protection, East Java. The research was conducted from January to April 2025 through bacterial isolation using the serial dilution method and fungal isolation using the baiting method, followed by macroscopic and microscopic observations as well as physiological and biochemical tests. The results showed that two soil bacterial isolates were obtained, in which one isolate was suspected to be Bacillus sp. with potential as a biological control agent, while the other isolate showed no potential as a biological control agent. A bacterial isolate initially suspected as Bacillus thuringiensis was not identified as an entomopathogenic bacterium based on physiological and biochemical tests. Fungal exploration resulted in isolates suspected as Trichoderma sp. and Aspergillus sp
EXPLORATION AND IDENTIFICATION OF MICROBES AND FUNGI FROM SOIL SAMPLES OF GUNGGUNG VILLAGE, BATUAN DISTRICT, SUMENEP REGENCY AS BIOLOGICAL CONTROL AGENTS AT THE EAST JAVA FOOD CROPS AND HORTICULTURE PLANT PROTECTION LABORATORY   Anindita Darma, Diva; Misnati Nur Indahsari, Wiwin
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/b4ev2f20

Abstract

Soil microorganisms play an essential role in maintaining soil fertility and suppressing plant pathogens, making them potential biological control agents in sustainable agriculture. This study aimed to explore and identify microbes and fungi from soil samples collected from yellow Kepok banana (Musa spp.) plantations in Gunggung Village, Batuan District, Sumenep Regency, as potential biological agents. The novelty of this research lies in the exploration of untreated Fusarium-infected banana plantation soil to obtain indigenous microbial isolates with biocontrol potential. A descriptive exploratory research design was employed. Bacterial isolation was conducted using the serial dilution method on Potato Dextrose Agar (PDA), followed by purification, macroscopic and microscopic characterization, 5% KOH test, soft rot test, and hypersensitivity test. Fungal isolation was performed using the baiting method with coconut, rice, and mealworms as substrates, followed by morphological identification. The results revealed the presence of Bacillus sp. as the dominant bacterial isolate, characterized by circular colonies, yellowish-white color, Gram-positive reaction, and rod-shaped cells. Soft rot and hypersensitivity assays indicated varying pathogenic responses among isolates. Fungal identification showed the presence of Aspergillus sp. (brownish-green, powdery colonies) and Penicillium sp. (yellowish-green, granular colonies). These findings indicate that soil from the study site contains microbial populations with potential applications as biological control agents. The study highlights the importance of exploring indigenous soil microorganisms to support environmentally friendly plant disease management and sustainable agricultural practices.
Co-Authors Anindita Darma, Diva Dyah Putri Yudha Yanti Fatimatul Ula, Siti