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All Journal The Health Researchers Journal
Wahyudi, Dhyani Paramita
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Public Health

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COMPARATIVE ANALYSIS OF INFARCT VOLUMES IN PERMANENT AND TRANSIENT MCAO MODELS USING TTC STAINING: A REVIEW OF CURRENT RESEARCH AND FUTURE DIRECTIONS Buana, Gherriandi Raja; Makkiyah, Feda Anisah; Wahyudi, Dhyani Paramita
The Health Researcher's Journal Vol. 2 No. 01 (2025): The Health Researchers Journal
Publisher : The SDGS Forum Communication

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.00000/js818616

Abstract

Ischemic stroke research often relies on animal models to understand the pathological processes underlying brain injury. Among the most widely used models are permanent middle cerebral artery occlusion (pMCAO) and transient middle cerebral artery occlusion (tMCAO), both of which simulate ischemic events in the brain. However, while existing studies have compared the effects of pMCAO and tMCAO on infarct volumes, many have been limited by short observation periods (e.g., 4 hours) or have focused only on non-occlusion models. This literature review aims to evaluate the comparative effectiveness of these two models in generating infarct sizes, specifically utilizing triphenyltetrazolium chloride (TTC) staining to measure infarct volumes. Our analysis highlights the variability in infarct volumes between the two models and emphasizes further research incorporating longer observation intervals (e.g., 6, 18, and 24 hours). This gap in research is crucial for refining experimental stroke models and improving the clinical relevance of findings, particularly regarding therapeutic strategies for ischemic stroke. The review also suggests potential research pathways to enhance model standardization and consistency in future studies, aiming for more accurate and reproducible results that will inform the development of effective stroke treatments.
A COMPARATIVE ANALYSIS OF TRANSCARDIAL PERFUSION TECHNIQUES FOR BRAIN FIXATION IN RODENTS: A CALL FOR STANDARDIZATION OF TRANSCARDIAL PERFUSION Wahyudi, Dhyani Paramita; Makkiyah, Feda Anisah; Buana, Gherriandi Raja
The Health Researcher's Journal Vol. 2 No. 01 (2025): The Health Researchers Journal
Publisher : The SDGS Forum Communication

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.00000/6nrjth51

Abstract

Transcardial perfusion is a crucial technique in rodent research for tissue fixation and preservation. The process typically involves flushing the vascular system with saline to remove blood, followed by fixation with 4% paraformaldehyde (PFA) or formalin. However, differences in species, procedural techniques, and fluid volumes can influence perfusion efficiency and tissue quality. This literature review compares six transcardial perfusion protocols used in mice and rats, focusing on procedural variations and fluid volume requirements. Data were collected from published protocols detailing perfusion techniques, obtaining evidence that substantial variability is observed in the protocols, including differences in anesthesia regimens, surgical approaches, perfusion techniques, fluid compositions, and fixation outcomes. Future research should focus on standardizing perfusion protocols to improve consistency in fixation quality, minimize tissue degradation, and preserve structural and molecular integrity.
Co-Authors Buana, Gherriandi Raja Makkiyah, Feda Anisah