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Perbandingan LC50 - 72 Jam terhadap Mortalitas Ikan Cere (Gambusia Affinis) pada Limbah Sungai, Laundry, dan Oli Bengkel Adinda, Diva; Putri, Irma Leilani Eka; Atifah, Yusni
Asian Journal of Science, Technology, Engineering, and Art Vol 2 No 1 (2024): Asian Journal of Science, Technology, Engineering, and Art
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajstea.v2i1.2395

Abstract

The cere fish species (Gambusia affinis) is a small fish, which is 2-3 cm. This fish is a wild fish that fills rivers, ditches and ditches. Cere fish are generally easy to adapt and reproduce. However, the water that irrigates rivers, ditches and ditches often contains community wastes, whether in the form of household waste, laundry, or even oil waste from workshops. So this study aims to obtain the LC5o-72 hour value of waste originating from river waste, laundry, and workshop oil on cere fish mortality. This research was conducted using a completely randomized design experiment. Each waste was treated in 4 treatments with different doses, namely 5 ml/L, 10 ml/L, 25 ml/L, and 50 ml/L for 72 hours with checking every 3 hours, 24 hours, 48 hours, and 72 hours. The results showed that laundry waste had a tolerance limit at a concentration of 10 ml/L and waste oil at a concentration of 25 ml/L.
Protective Effects of Apple Peel Extract (Malus sylvestris Mill.) on the Sperm Quality and Testicular Histology in Mice Borax-Induced Atifah, Yusni; Adinda, Diva; Yuliati, Netri
Jurnal Riset Biologi dan Aplikasinya Vol. 7 No. 1 (2025)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v7n1.p20-30

Abstract

Borax, a toxic material, can disrupt the testes, hypothalamus, and spermatogenesis in the male reproductive system, leading to infertility. Apple peel contains quercetin, a substance crucial for boosting antioxidant levels and preventing free radical damage. The study aimed to assess the impact of apple peel extract on spermatozoa quality and testicular histology in borax-induced mice. The study utilized a Complete Randomized Design (CRD) with five treatment groups and five replications of each group: C- (without treatment), C+ (Borax), T1, T2, and T3, where borax was administered alongside various doses of apple peel extract: 0,2 mg, 0,4 mg, and 0,8 mg respectively. Testicular sections were collected for histopathological analysis and caudal epididymal sections for sperm quality assessment. Data were analyzed using ANOVA and DMRT tests using SPSS 23. The results showed that apple peel extract significantly increased the quality of mice spermatozoa in borax-induced mice (p<0.05). Apple peel extract to borax-induced mice also positively affected testicular diameter and weight, as well as the diameter of the seminiferous tubules and the number of Leydig cells. The optimal dose of apple peel extract in improving the quality of spermatozoa and testicular histology of mice was found to be0.4 mg / 20g BW.