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Numonov Otabek Urmonovich
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Automotive Engineering Computer Science & IT Control & Systems Engineering Engineering Industrial & Manufacturing Engineering Materials Science & Nanotechnology

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Comparative characteristics of the leaving of glutathione from cells of different types. Melanova Nazira Rashidovna; Numonov Otabek Urmonovich
International Journal on Orange Technologies Vol. 2 No. 10 (2020): October
Publisher : Research Parks Publishing LLC

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31149/ijot.v2i2.712

Abstract

Glutathione (GSH) is a negatively charged tripeptide, which is a major determinant of the cellular redox state and defense against oxidative stress. It is assembled inside and degraded outside the cells and is released under various physiological and pathophysiological conditions. The GSH release mechanism is poorly understood at present. In our experiments, freshly isolated rat thymocytes were found to release GSH under normal isotonic conditions at a low rate of 0.8260.07 attomol/cell/min and that was greatly enhanced under hypoosmotic stimulation to reach a level of 6.160.4 attomol/cell/min. The swelling-induced GSH release was proportional to the cell density in the suspension and was temperature-dependent with a relatively low activation energy of 5.460.6 kcal/mol indicating a predominant diffusion mechanism of GSH translocation. The glutathione release rate from rat thymocytes was found to be 4-10 times higher compared to the human red blood cells. Cultured melanoma cells also exhibited substantial release of glutathione both in normal and hypoosmotic stress conditions
Strip Till Age of Soil for Deuteric Sowing (Second Crop) Nasritdinov Akhmadzhon Abdukhamidovich; Muhabbat Davlatova Urmanovna; Numonov Otabek Urmonovich
International Journal on Orange Technologies Vol. 3 No. 4 (2021): IJOT
Publisher : Research Parks Publishing LLC

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31149/ijot.v3i4.1636

Abstract

According to the test results, a section of the working bodies of strip tillage for re-sowing was selected, the parameters of the height, length of the soil-shifting plate of its flat-cutting paw and the angle of attack of the discs were experimentally determined.
Co-Authors Melanova Nazira Rashidovna Muhabbat Davlatova Urmanovna Nasritdinov Akhmadzhon Abdukhamidovich