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All Journal Indonesian Journal Health Science and Medicine Journal of Medical Genetics and Clinical Biology (JMGCB)
Abas, Ismaal Jmia
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Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary

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MrKD Gene in Environmental Isolates of Biofilm Producing Klebsiella Pneumoniae: Gen MrKD pada Isolat Lingkungan Penghasil Biofilm Klebsiella pneumoniae Hamady, Sabah Raheem; Abas, Ismaal Jmia
Indonesian Journal on Health Science and Medicine Vol. 2 No. 1 (2025): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/ijhsm.v2i1.77

Abstract

Biofilm This concept includes cell aggregates that are not attached to an interface but share traits with biofilms, such as flocs (floating biofilms) and sludge.K. pneumonia is a member of the Enterobactericeae family and is Gram-negative, non-motile, facultatively anaerobic, lactose fermentation, and is present in the environment in places like soil, vegetation, and water. It is also easily isolated from the mucosal surfaces of mammals. K. pneumoniae has at least two variants of type 3 fimbriae, with the most diversified form being the mrkD gene. Materials and Methods: Biochemical and Api20E assays were used to identify K. pneumoniae isolated from different environmental samples in Basra Governorate, Iraq, from 1/11/2021 to 1/1/2022. The Kirby-Bauer assay was performed, and biofilm phenotype formation was evaluated. Finally, the mrkD gene was detected by the PCR method. Results: results showed that the number of K.pneumoniae bacteria was 23 out of 122 samples, and then it was revealed that its ability to form biofilm was used in this study, where the two methods of Congo red were used. The presence of the mrkD gene, which is thought to be responsible for biofilm production, was investigated using a polymerase chain reaction device, and the mrkD gene was found in 34.78% of the samples. Conclusion: This research highlights that the presence of the mrkD gene in K.pneumoniae bacteria, which was isolated from different environmental samples, has a relationship with biofilm formation and shows the extent of biofilm resistance to antibiotics Highlights: Biofilm & K. pneumoniae: Forms biofilms, contains mrkD gene, resists antibiotics. Methods: Identified via biochemical tests, Kirby-Bauer assay, and PCR analysis. Results: 34.78% of isolates carried mrkD gene, linked to biofilm formation.. Keywords: Klebsiella pneumoniae, biofilms, mrkD gene.
ANTIBIOFILM SILVER NANOPARTICLES PRODUC BY ESCHERICHIA COLI abdulrazz, Mohammed aqeel; Abas, Ismaal Jmia
Journal of Medical Genetics and Clinical Biology Vol. 1 No. 7 (2024): Journal of Medical Genetics and Clinical Biology
Publisher : PT. Antis International Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61796/jmgcb.v1i7.747

Abstract

coli bacteria were obtained from the soil near the Euphrates River in the northern region of Basra City and cultured on nutrient agar media. The bacterial isolate was subjected to purification and thereafter seen under a microscope. The study revealed that E. coli bacteria has the capacity to synthesize silver nanoparticles when combined with a 1 mM AgNO3 solution. The synthesis of AgNPs was deduced by examining the change in color of the reaction mixture. The UV-vis spectroscopy results indicated that the silver nanoparticles exhibited absorbance at a specific wavelength of 428 nm. Additionally, the FT-IR analysis revealed the presence of active groups in the silver particles, which played a role in maintaining their stability. The SEM and TEM studies revealed that the AgNPs exhibited a spherical morphology, with diameters ranging from 47.66 to 11.68 nm. A cubic crystal structure of silver was confirmed using an X-ray diffraction examination, while an EDX test indicated that the final product was silver (Ag). The antimicrobial efficacy of silver nanoparticles (AgNPs) against bacteria isolated from diseased patients was evaluated using the Well diffusion agar technique. It was shown that nano-silver effectively inhibited the development of the bacteria.The study utilized 96 well Microtiter plates to assess the inhibitory effect of AgNPs on biofilm development in harmful bacteria.
Co-Authors abdulrazz, Mohammed aqeel Hamady, Sabah Raheem